Qs 41-50

Question 1

Ouchterlony’s precipitation is used:

Answer 1

Only for determination of antigen or antibody precence.

Question 2

Which immune diffusion assays is for quantitative determination of antigen?

Answer 2

Single radial immunodiffusion.

Question 3

The main deficiency of quantitative assays based on precipitation is:

Answer 3

Lack of sensibility

Question 4

The difference between agglutination and precipitation is in:

Answer 4

-Size
-Solubility
-Location of antigen

Question 5

The titer of antibody is:

Answer 5

Highest dilution of an antiserum that still react with antigen in serological reactions.

Question 6

In a direct ELISA for testing of antibody, the intensity of measured after adding the substrate in final step is:

Answer 6

Inversely proportional to the concentration of antibody in patient serum.

Question 7

What is main purpose of respiratory burst in phagocytes?

Answer 7

Creation of oxidative radicals which destroy ingested material.

Question 8

For determination of number of leukocytes the blood is?

Answer 8

diluted in turk solution (1:20)

Question 9

Size of the cells in flow cytometer is detected by?

Answer 9

Forward scatter.

Question 10

Polymononuclear cells are:

Answer 10

-neutrophils
-basophils
-eosinophils