Questions on practice tests

Question 1

How do confocal microscopes work?

Answer 1

Use a laser to scan samples that have been dyed

Question 2

How do bright field microscopes work?

Answer 2

Have objectives above stage

Question 3

If a plasmid has 3 sites for a restriction enzyme, how many fragments will it have?

Answer 3

3

Question 4

Electroporation

Answer 4

Used to transform bacteria

Question 5

PCR optimal conditions

Answer 5

Priner, DNA, dNTPs, and Buffer kept constant- temp 75-95 C

Question 6

Which of the following should NOT be used to kill DNA?
1. UV light
2. Bleach
3. Alcohol

Answer 6

Alcohol

Question 7

Enzymes work best in a (wide/narrow) pH range

Answer 7

Narrow

Question 8

Which will give you better seperation on DNA running bands?
1. Higher % agarose gel
2. Lower % agarose gel
3. + voltage, - running time
4. + voltage, _ running time

Answer 8

Higher % agarose gel

Question 9

In gel electrophoresis, bigger bands move to the (top/bottom) of the gel

Answer 9

Top

Question 10

Which can be used to deliver new DNA to a cell?
1. Lipid
2. Plasmid
3. Virus
4. All

Answer 10

All of the above

Question 11

What is used to amplify DNA in PCR?
1. Phosphate
2. Restriction enzyme
3. Ligase
4. DNA polymerase

Answer 11

DNA polymerase

Question 12

Can monoclonal anitbodies be produced in human cells?

Answer 12

Yes

Question 13

Why is there a blocking step in a Western Blot?

Answer 13

To prevent non specific binding of antibodies used for detection

Question 14

What type of polymerase is used for PCR?

Answer 14

Heat-stable DNA polymerase

Question 15

What is a horizontal gel electrophoresis box used for?

Answer 15

Running DNA gels

Question 16

What is a vertical gel electrophoresis box used for?

Answer 16

Protein gels

Question 17

What does SDS page determine?

Answer 17

Size/purity of a specific protein

Question 18

What is the time a protein stays active called?

Answer 18

Stability

Question 19

What is the level of protein activity called?

Answer 19

Activity

Question 20

What type of respiration uses O2 as a raw material?

Answer 20

Aerobic

Question 21

What type of respiration is a process where glucose is used by a cell?

Answer 21

Aerobic and anaerobic

Question 22

What type of respiration releases CO2 and ethyl alcohol?

Answer 22

Anaerobic respiration

Question 23

What type of respiration uses CO2 as a raw material?

Answer 23

Neither

Question 24

Prokaryotic DNA structure

Answer 24

Long, circular, 1000s o genes

Question 25

What is semi-conservative replication?

Answer 25

The process in DNA replication where each original strand serves as a template for a new strand

Question 26

When does transcription begin?

Answer 26

When RNA polymerase binds to the promoter on a DNA strand

Question 27

What enzyme is used in DNA replication?

Answer 27

DNA polymerase

Question 28

Where does translation take place in prokaryotes?

Answer 28

Ribosomes

Question 29

If a bacterial cell didn't contain restriction enzymes, what would happen?

Answer 29

It would be easily lysed and infected by bacteriophages.

Question 30

Complementary base pairing between 2 DNA structures is due to formation of what?

Answer 30

Hydrogen bonds

Question 31

What does cellulose do?

Answer 31

Only provides energy storage and structure in cells

Question 32

Which of the following could use sugar to produce energy without O2? 1. Bacterial 2. Yeast 3. Animal (muscle) 4. All of the above

Answer 32

All of the above

Question 33

Where does protein synthesis happen?

Answer 33

The cytoplasm

Question 34

What is an epitope?

Answer 34

The part of an antigen recognized by the immune system

Question 35

A tech performs an ELISA using primary antibody with mouse Ig62a isotype to analyze patient blood samples. Which would be best? 1. Mouse anti-human 2. Mouse anti-rabbit 3. Human anti-rabbit 4. Rabbit anti-mouse

Question 36

(True/False)- Shaking isn't necessary to oxygenate aerobic liquid culture for optimal growth.

Answer 36

False

Question 37

A tech is asked to determine the size of a PCR product, and runs it on a gel. The PCR band is well above (much closer to wells) than the DNA ladder bands. How should the tech proceed determining the size of a PCR product the next time samples are run? 1. Run gel at a lower voltage 2. Run gel at higher voltage 3. Use a DNA ladder with larger fragments 4. Use a DNA ladder with smaller fragments

Answer 37

3

Question 38

Is a plasmid has 2 bands, how many restriction sites does it have?

Answer 38

2

Question 39

What event caused the advent of recombinant DNA technology?

Answer 39

Isolation of restriction enzymes

Question 40

What are the steps to launch a new drug?

Answer 40

Identifying product, research and development, clinical trials, manufacturing, sales

Question 41

What are the steps to bring a biopharm product to market?

Answer 41

Research and development, process development, small scale manufacturing, large scale manufacturing

Question 42

Who regulates cGMPs?

Answer 42

FDA

Question 43

What does QA handle?

Answer 43

maintaining and controlling documentation

Question 44

What is Assay Validation?

Answer 44

Providing documented evidence that a method works

Question 45

What is the proper way to correct an incorrect date on paperwork?

Answer 45

Cross out with a single line, initial and date it

Question 46

What does a water bath do?

Answer 46

Regulate the temperature of anything subjected to heat

Question 47

Why would you only want to change part of a viral, interacting molecule on a cell?

Answer 47

To allow it to retain its original functions

Question 48

(True/false) All biohazard waste must be biologically inactivated before being disposed of as regular trash.

Answer 48

True