Quiz 1

Question 0

BSL 2

Answer 0

• Can cause disease in healthy people, but organisms are easily contained
• Limited lab access and biohazard warning signs
• Gloves, lab coat, eye protection or face shield
• Access to autoclave

Question 1

BSL 1

Answer 1

• No health risk
• Primary barriers: none
• Secondary barriers: Open bench tops & sinks

Question 2

BSL 3

Answer 2

• Can cause severe disease especially when inhaled
• Controlled access to lab, no unsterilized material can leave lab, clothes need decontamination
• Bio safety cabinets used for manipulation
• HIV

Question 3

BSL 4

Answer 3

• Highly virulent microbes with extreme risk to humans
• Clothing needs to be changed for practice, everything decontaminated
• Isolated building or lab
• EBOLA

Question 4

Negative air flow

Answer 4

Air flows in preventing contamination of the surrounding environment

Question 5

Gloves, glasses, lab coat

Answer 5

Protect user against spills/splashes of them or someone else

Question 6

Vaccination of workers

Answer 6

Protects from infection in the event that other protective measures fail

Question 7

Foot pedal

Answer 7

Prevents passing of infections as the sink handle acting as a common vehicle for infections

Question 8

No eating or drinking in lab

Answer 8

Keep potentially contaminated food and finger away from the mouth

Question 9

How to dispose of

• Petri dish with fungus
• glass culture with bacteria
• needle to draw blood

Answer 9

• tape close & put in white buckets
• cleanse of marks/tape and put on test tube rack in back of room
• put in hard sided biohazard container (box)

Question 10

Locations of

• fire extinguisher
• disinfectant
• first aid kit
• bio hazardous waste disposal
• sharps container

Answer 10

• by door
• wide lab benches
• top of fridge
• white buckets and test tube rack
• by door

Question 11

Parfocal

Answer 11

Ability to maintain focus when switching between objectives

Question 12

Magnification

Answer 12

Creation of a larger than life image

Question 13

Resolution

Answer 13

Clarity of an image produced by a set of lenses

Question 14

Contrast

Answer 14

Degree of difference between the lightest and darkest parts of an image

Question 15

Ocular micrometer

Answer 15

Circular glass disc that has a series of regularly spaced markings etched onto its upper surface

Question 16

How should the condenser and diaphragm be adjusted for optimum viewing?

Answer 16

Condenser: best at its highest point
Diaphragm: almost completely closed

Question 17

Ocular

Answer 17

Lens closest to your eyes when looking through a microscope, 10x

Question 18

How to properly clean the lenses on a microscope

Answer 18

• Use lint free tissues moistened with an appropriate solvent
• Use circular motion working outward
• Clean immersion oil from objective lens

Question 19

Resolution formula

Answer 19

Resolution = .61• wavelength /

Numerical aperture

Question 20

Parcentric

Answer 20

Centered specimens will remain in view when switching magnifications

Question 21

Depth of field

Answer 21

Distance from lens that is in focus

Question 22

How to know if something is resolvable

Answer 22

If distance between points is higher than it’s resolution, then yes

Question 23

Nosocomial

Answer 23

Acquired through a health care facility

Question 24

Aseptic

Answer 24

Free of viable microbes

Question 25

Sterile

Answer 25

Free of all viable life forms such as viruses and prions | no growth on agar

Question 26

Ubiquitous

Answer 26

Existing everywhere at the same time

Question 27

Pathogen

Answer 27

A microorganism causing disease

Question 28

Inoculation

Answer 28

Implantation of microorganisms into a culture media

Question 29

Turbid

Answer 29

Cloudy appearance of a nutrient solution in a test tube due to growth of a microbial population

Question 30

Potable

Answer 30

Fit to drink, clean and odor free

Question 31

How would you recognize microbial growth in a liquid media? Solid media?

Answer 31

Liquid: cloudiness of broth Solid: formation if discrete colonies of cells on the surface

Question 32

What is a colony and it's relationship to a cell?

Answer 32

- Colony: discrete collection if many thousands to millions of cells - All thousands of cells in a colony are descended from a single cell

Question 33

What additional info does a solid culture have compared to liquid?

Answer 33

Growth in a solid media indicates the bacterial density of the sample

Question 34

Characteristics of bacterial and fungal growth

Answer 34

Bacterial: typically smaller, circular, white/creamy/yellow, wet Fungal: leathery, cottony, powdery, diverse colors

Question 35

Loop

Answer 35

To transfer bacteria from one medium to another

Question 36

Needle

Answer 36

To transfer bacteria by stabbing it into another medium

Question 37

Sterile swabs

Answer 37

To collect from the environment

Question 38

Broth, Agar

Answer 38

``` Liquid medium Solid medium (petri dishes) ```

Question 39

Labeling a plate

Answer 39

Initials, date, class time, TSA, area of swab

Question 40

Formula for total magnification

Answer 40

Total mag= ocular mag(10x) • objective magnification