Quiz 2

Question 1

Trismus

Answer 1

Inability to open mouth fully

Question 2

Aerobic

Answer 2

Living and growing int the presence of oxygen

Question 3

Anaerobic

Answer 3

Growing best with the absence of oxygen

Question 4

Sequelae

Answer 4

Morbid complication that follows a disease

Question 5

Colony

Answer 5

Macroscopic cluster of cells appearing on a solid media each arising form the multiplication of a single cell

Question 6

What is the difference between a pure culture and a mixed culture?

Answer 6

Pure culture contains microbial species while a mixed contains multiple

Question 7

How can a pure or mixed culture become contaminated? How can this be prevented?

Answer 7

If your loop touches something else

• Improper sterilization or airborne contaminates
• Prevent by proper aseptic techniques

Question 8

What is the importance of generating isolated bacterial colonies?

Answer 8

Is is the only way to obtain a PURE culture to see what microbial effects the organism has

Question 9

Subculture

Answer 9

A second-generation culture from a well established colony of organisms

Question 10

Cellular morphology

Answer 10

Shape (rod, spirilla)

Question 11

Colonial morphology

Answer 11

Configurations, margins, elevation

Question 12

Configuration
Margin
Elevation

Answer 12

• Entire colony
• Colony Edge
• Look from the side

Question 13

Morphology

Answer 13

Study of organismic structure

Question 14

Arrangement

Answer 14

The manner in which bacterial cells associate with one another

Question 15

Pleomorphism

Answer 15

Normal variability of cell shapes in a single species

Question 16

Mordant

Answer 16

Chemical that fixes dye in or on cells by forming an insoluble compound and promoting retention of the dye

Question 17

Streptococci

Answer 17

OOOOOOOOO

Question 18

Staphlococci

Answer 18

OOOOOOO
OOOOO
OOOOOOO
OOOOO

Question 19

Tetrad

Answer 19

OO

OO

Question 20

What stains are good for negative staining?

Answer 20

Useful with negatively charged bacterial cells which repel the dye (dark background)

Question 21

Which stains are good for simple staining?

Answer 21

Useful with positively charged particles (light background)

Question 22

Simple Stain

Answer 22

Using one dye to create contrast between bacteria and the background

Question 23

Differential Stain

Answer 23

Using multiple dyes to create contrast between bacteria and the background

Question 24

What two stains are appropriate to find the shape and arrangement of bacterial species?

Answer 24

Negative and simple staining

Question 25

Primary Stain

Answer 25

Crystal violet

Question 26

Mordant - stain

Answer 26

Iodine

Question 27

Decolorizer

Answer 27

Ethyl alcohol

Question 28

Counter stain

Answer 28

safranin

Question 29

Order of Gram stain

Answer 29

1. Primary Stain 2. Mordant 3. Decolorizer 4. Counterstain

Question 30

Gram + is | Gram - is

Answer 30

- Purple | - Pink

Question 31

Gram reaction

Answer 31

Appearance of a cell after being gram stained

Question 32

Acid fast stain

Answer 32

1. Carbol Fuscian 2. Heat 3. Acid Alcohol 4. Methylene Blue

Question 33

Endospore Stain

Answer 33

1. Malach Ite Green 2. Heat 3. Water 4. Safranin

Question 34

Bronchoscopy

Answer 34

Procedure in which a long, thin tube is inserted through the mouth to examine the airway to the lungs

Question 35

Etiological agent

Answer 35

Microbial cause of disease (pathogen)

Question 36

Bilateral infiltrates

Answer 36

Abnormal pulmonary opacity, indicative of fluid or cells found in both lungs

Question 37

Prophylactic

Answer 37

Any device, method or substance used to prevent disease

Question 38

2 diseases cause by Acid fast bacteria and what kind of bacteria is it

Answer 38

MYCObacterium- has micolic acid in cell wall - MYCObacterium - MYCOlic acid - ACID fast

Question 39

Why is a gram stain generally done prior to an acid fast or endospore stain?

Answer 39

If a cell stains Gram-, there is no need for wither of the stains because those are both for gram +