Random Bits and Bobs

Question 1

Fontana stain

Answer 1

+ve for melanin – Melanoma

Question 2

Congo red stain

Answer 2

apple
green birefringence under polarised light +ve for amyloid – Amyloidosis

Question 3

Rhodanine stain

Answer 3

Golden brown against blue counterstain

+ve for copper – Wilson’s disease

Question 4

Prussian blue

Answer 4

+ve for iron – Haemachromatosis

Question 5

Perl’s stain

Answer 5

exact same
as Prussian blue +ve for iron – Haemachromatosis

Question 6

Cytokeratin

Answer 6

+ve for epithelial cells – Carcinoma

Question 7

CD45

Answer 7

+ve for lymphoid cells – lymphocytes

Question 8

Ziehl-Neelson

Answer 8

Red against a blue background
+ve for acid-fast bacilli – TB (diagnostic)

Question 9

Rhodamine-Auramine
stain

Answer 9

Bright yellow – TB (screen for TB but not diagnostic)

Question 10

Gomori’s methanamine
silver stain

Answer 10

Flying saucer shaped cysts – Pneumocystis jirovecii

Question 11

Modified Kimyoun acid
fast stain

Answer 11

+ve for Cryptosporidium parvum

Question 12

India ink stain

Answer 12

Yeast cells surrounded by halos – Cryptococcus neoformans

Question 13

Giemsa stain

Answer 13

Cytoplasmic inclusions – Chlamydia psittaci

Question 14

Fite stain

Answer 14

+ve for Mycobacterium leprae