Rate of an Enzyme Controlled Reaction ( Topic 1)

Question 1

What factors affect the rate of enzyme-controlled reactions?

Answer 1

1. Temperature.
2. pH.
3. Substrate concentration.
4. Enzyme concentration.
5. Inhibitors.

Question 2

What could be the control variables for this experiment, and how could they be controlled?

Answer 2

1. The volume of the substrate solution.
2. The volume of the enzyme solution.
3. The concentration of the substrate or enzyme solution.
4. The temperature ( Keep the test tubes in a water bath.)
5. pH ( Place a buffer into the solution)

Question 3

How would you calculate the reaction using data?

Answer 3

1. Find the mean time for the reaction to stop.
2. The mean ratio of reaction is 1/mean time.

Question 4

What are some limitations in this experiment?

Answer 4

1. The endpoint, such as a colour change, is subjective, which can lead to the data being inaccurate.
2. The range of values tested may not be wide enough to be representative.
3. Other factors may affect the rate of reaction.
4. If not enough intermediate values are tested, identifying the optimum conditions won’t be possible.