Recombinant DNA Techniques Flashcards
(8 cards)
1
Q
Southern blotting is used to identify?
A
Size restriction fragments in complex DNA mixture
2
Q
Northern blot is used to identify?
A
mRNA size in complex mixture of DNA/RNA hybridization
3
Q
In ______ chromosomal clones are arrayed and can be used for analysis of deletions or duplications
A
CGH
4
Q
__________ are extrachromosmal circular elements in bacteria, carrying?
A
Plasmids, antibiotic resistance genes
5
Q
cDNA libraries are made from ________ which has been converted to cDNA with help of?
A
mRNA, reverse transcriptase and RNaseH
6
Q
RISC complex binds to?
A
Target mRNA
7
Q
Argonaut, a component of RISC complex does what?
A
Makes cut in target mRNA leading to rapid degradation of mRNA
8
Q
Restriction enzymes are _____________ for dsDNA and are sequence specific
A
Endonucleases
