Reproduction In Plants Flashcards
(113 cards)
Whats vegetative propagation?
Type of asexual reproduction in which new plants develop from vegetative part of plant.
Concept of tissue culture was given by
Haberlandt.(1901)
Whats tissue culture?
Growth of explant in vitro conditions.
Growth of cells, tissues, organs in lab conditions.
Whats explant?
Cells, tissue or organs that is used to start culture technique.
Loading of explant over nutrient medium is called
Innoculation.
Closed chamber used to conduct tissue culture is c/d
Bio-reactor or innoculation chamber.
Nutrient medium should have
Minerals
Sucrose (c12h22o11)(glu-glu)
Phytohormones esp. auxin and cytokinins
Most commonly used nutrient medium is
MS medium.
Whats liquid medium?
Prepared by dissolving all contents with water.
Aka suspension medium.
Whats solid/gel medium?
Prepared by adding 1-2% agar-agar in liquid medium.
Sterilisation is done by
Boiling or treatment with alcohol.
Surface sterilisation is done by
Adding anti-microbials like chlorax.
UV sterilisation is done by
Passing uv radiations in a bio-reactor.
Whats continuous culture?
When older medium is replaced by new fresh medium.
Whats batch culture?
When cells are shifted to new medium.
Nucellus culture is done so as to
Produce disease free plants.
Give 2 significance of embryo culture?
To overcome problem of seed dormancy.
To perform embryo rescue (to prevent embryo from forming non-viable seeds).
Production of virus free plants is obtained by ______ culture.
Meristem culture.
Whats androgenic haploid?
Haploid plants developed from anther cells(male).
Which type of tissue culture results in production of androgenic haploids?
Anther culture.
Whats cybrid?
Cybrid / somatic hybrid =organism produced by crossing two genetically organisms asexually.
Whats micropropagation /somo-clonal propagation?
Type of tissue culture in which a new plant is produced from small group of cells.
Production of cybrid is a significance of _________ culture.
Protoplast culture (fused protoplast is used as explant).
Virus used in protoplast culture for fusion of protoplast of 2 cells is ______ .
Sendai virus (used in viral fusion of protoplast).