Required Practical 1- Enzyme Controlled Reactions

Question 1

How to set up control in practical measuring enzyme activity?

Answer 1

Replace enzyme solution with distilled water or boiled enzyme solution

Question 2

How to find initial rate of reaction from practical

Answer 2

Plot results on graph of rate of reaction against time.
Draw a tangent at time= 0 to find initial rate

Question 3

Factors affecting rate of enzyme controlled reactions?

Answer 3

-Temperature
-concentration of substrate solution
-concentration of enzymes solution
-pH

Question 4

What are buffer solutions used for

Answer 4

To maintain pH to prevent breaking hydrogen bonds in secondary/tertiary structure- denature

Question 5

Why are test tubes left in water baths before adding enzymes?

Answer 5

To allow solutions to reach given temperatures, keep constant (equilibriate temp)

Question 6

Why is colorimeter used?

Answer 6

Provides quantitative result
-more accurate (not subjective)
-easier to make comparisons

Question 7

Why is buffer solution used?

Answer 7

Maintain constant pH

Question 8

Why are solutions mixed gently before placed in colorimeter?

Answer 8

Avoid formation of bubbles or froth- could significantly affect absorbance reading on colorimeter

Question 9

Why is it important never to touch sides of cuvette

Answer 9

Fingerprints affect absorbance reading on colorimeter