restriction endoluceases Flashcards

1
Q

names of enzymes

A

restriction endonuclease

restriction enzymes

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2
Q

what do RE do

A

there are many RE

each one recognises a specific palindromic sequence

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3
Q

stages of restriction enzyme

A

enzyme cut a DNA molecule at the specific palindromic sequence - recognition sequence

breaks phosphodiester bonds between adjacent nucleotides

generates sticky ends

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4
Q

sticky ends

A

unpaired bases that can form H-bonds with complimentary bases

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