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Review Sheet for Basic Lab Skills Flashcards

(65 cards)

1
Q

Micropopettor P20

A

Purpose: Accurately measure volumes less than 20 uL
Range measured:2-20uL
Maximum allowed: 20 uL

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2
Q

Micropipettor p10

A

Purpose: Accurately measures less than 10 uL
Range measured: 1-10 uL
maximum Allowed: 10uL

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3
Q

Hot Plate/Stirrer

A

Purpose: to heat chemicals solutions and mix sing stir bar
Range Measured: N/A
Maximum allowed: N/A

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4
Q

Micropipettor p200

A

Purpose:Accurately measure volumes less than 200uL
Range Measured:20-200uL
maximum Allowed:200uL

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5
Q

Micropipettor 1000

A

Purpose:Accurately measure volumes less than 1000uL
Range Measured:100-1000uL
Maximum Allowed:1000uL

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6
Q

Beaker

A

Purpose: Holds liquids
Range Measured: N/A
Maximum Allowed: N/A

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7
Q

Flasks

A

Purpose: Holds Solutions
Range Measured: N/A
Maximum Allowed/ N/A

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8
Q

Graduated Cylinders

A

Purpose: Measures out liquids
Range Measured: 5-1000mL
Maximum Allowed: 1000mL

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9
Q

Water Bath

A

Purpose: Heats up liquids
Range Measured: N/A
Maximum Allowed: N/A

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10
Q

Pipet aides

A

Purpose: Measures the solutions
Range Measured: 0.1-100mL
Maximum: Allowed:100mL

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11
Q

Bunsen Burners

A

Purpose:a small adjustable gas burner used in laboratories
Range Measured: N/A
Maximum Allowed: N/A

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12
Q

Vortexer

A

Purpose: Used more mixing solutions
Range Measured: N/A
Maximum Allowed: N/A

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13
Q

Heat block

A

Purpose: Heats up samples
Range Measured: N/A
Maximum Allowed: N/A

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14
Q

Incinerator

A

Purpose: Kills Bacteria
Range Measured: N/A
Maximum Allowed: N/A

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15
Q

Buret

A

Purpose: used in quantitative chemical analysis to measure the volume of a liquid or a gas
Range Measured: 1-50mL
Maximum Allowed: 50mL

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16
Q

Microcentrifuge Tube

A

Purpose: Spins the Liquids
Range Measured: N/A
Maximum Allowed: N/A

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17
Q

Electronic Balance (Red)

A

Purpose: Measures out the exact amount you need
Range Measured: N/A
Maximum Allowed: N/A

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18
Q

Electronic Balance(0.01)

A

Purpose: Measures masses of samples
Range Measured: 0.01g
Maximum Allowed: 0.01g

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19
Q

Electronic Balance(0.001)

A

Purpose: Measures masses of samples
Range Measured: 0.001g
Maximum Allowed: 0.001g

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20
Q

Incubator

A

Purpose: grows the bacteria
Range Measured: N/A
Maximum Allowed: N/A

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21
Q

Parafilm

A

Purpose: To sill of the bacteria
Range Measured: N/A
MAximum Allowed: N/A

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22
Q

Microcentrifuge tube

A

Purpose: processing and storage of small volume samples, extracts, and reagents
Range Measured: N/A
Maximum Allowed: N/A

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23
Q

15 mL conical tube

A

Purpose: Tubes used to hold liquids
Range Measured:15mL
Maximum Allowed: 15mL

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24
Q

50 mL conical tube

A

Purpose: Tube used to hold liquids
Range Measured: 50mL
Maximum Allowed: 50mL

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25
1 mL Serological pipets
Purpose: Measures out the accurate amount of the solutions Range Measured: 1mL Maximum Allowed: 1mL
26
5 mL Serological pipets
Purpose:Measures out the accurate amount of the solutions Range Measured: 5mL Maximum Allowed: 5mL
27
10 mL Serological pipets
Purpose: Measures out the accurate amount of the solutions Range Measured: 5mL Maximum Allowed: 5mL
28
25 mL serological pipets
Purpose: Measures out the accurate amount of the solutions Range Measured: 25mL Maximum Allowed: 25mL
29
Peri dish
Purpose: Used for making agar plates Range Measured: N/A Maximum Allowed: N/A
30
Eye Wash
Purpose: Cleans out the chemicals that go into your eyes Range Measured: N/A Maximum Allowed: N/A
31
Inoculation loop
Purpose: Used for Streaking Bacteria Range Measured: N/A Maximum Allowed: N/A
32
Body drench
Purpose: Cleans the chemicals off your body Range Measured: N/A Maximum Allowed: N/A
33
Spectrophotometer
Purpose: Measures the intensity of light Range Measured: N/A Maximum Allowed: N/A
34
Spatula
Purpose: mixing, scraping, and other tasks related to transferring materials and samples from one place to another. Range Measured: N/A Maximum Allowed: N/A
35
pH meter
Purpose: Determines if something is an acid, base, or neutral Range Measured: 1-14 Maximum Allowed: 14
36
Caustic
able to burn organic tissue by chemical action
37
Toxic
can harm or kill if swallowed
38
flammable
can catch fire if not used right
39
Chronic
continuous or repeated contact with a toxic substance over a long period of time
40
Acute
chemicals that may be fatal as a result of a single exposure or exposure of a short duration.
41
Flash point
the temperature at which a particular organic compound gives off sufficient vapor to ignite in air.
42
FDA
Food and Drug Administration
43
EPA
Environmental protection agency
44
OSHA
Occupational safety and health administration
45
NFPA
National Fire Protection Association
46
Fine adjustment knob
Fine focus the image when viewing at a higher magnification
47
Coarse adjustment knob
allows quick focusing by moving the object lens/stage up and down
48
Coaxial adjustment knob
allows the user to change between the knobs by feel
49
ocular lens
magnifies the image produced by the microscopes objective
50
objective lens
unit that determines the basic performance/function of an optical microscope
51
DI water (dH2O)
Removal of Ions
52
Immersion Oil
increases the resolving power of the microscope by replacing the air gap between the immersion objective lens and cover glass with a high refractive index medium and reducing light refraction.
53
Analyte
A substance whose chemical constitution are being identified or measured
54
Titrant
A Substance that is added to Titration
55
Titration
Solution of known concentration is used to determine te concentration of an unknown soluton
56
PPE
Personal Protection Equipment
57
Aseptic technique
Using practices and procedures to prevent contamination from Pathogens
58
Acidic
Having properties of and acid
59
Alkaline
having properties of an alkali/pH greater than 7
60
Hydroxide
compound of a metal with the group -OH
61
Concentrated to Dilute
prepare 1000mL of 0.5 HCI using a 1.0 M HCI (1.0)(V1)=(1000)(0.5) V1=500
62
Ratio Dilution
The urinalysis test requires volumes of 1mL. You need to dilute the samples 1:2, 1:5, and 1:10 9Urine water). What are the volumes 9mL0 of the urine and the volume of the water? Vf/Df-Vc
63
% solution
a 10% bleach solution of 500 mL is nned. What is the volume (mL) of bleach that you need to add to make the solution (x/100)x(Vf)=ms
64
Molar Solution
We need 1000mL of a 2.5 M salt solution. The molecular weight of the salt is 75g. What is the mass (g) that you need to add? M=ms/M.W
65
Serial Dilution
1:1,1:10,1:100,1:1000,1:10,000