Rh typing

Question 1

Most common Rh type in white population

Answer 1

1. R1 = CDe (42%)
2. r = cde (37%)
3. R2 = cDE (14%)

Question 2

Most common Rh type in black population

Answer 2

1. Ro = cDe (44%)
2. r = cde (26%)

Question 3

Most common Rh type in Asian population

Answer 3

1. R1 = CDe (70%)
2. R2 = cDE (21%)

Question 4

How is Rh typing performed on gel?
Steps

Answer 4

Using gel
1. Add 10uL of the (3-5%) patient RBC sample to all 6 microtubules.
2. Centrifuge for 10mins in ID-Centrifuge
3. Read and record results.

Question 5

How is Rh typing performed in tubes?
Steps

Answer 5

1. Set up rack correctly
2. Add 1 drop of Anti-D/C/c/E/e/K into tubes.
3. Add 1 drop of test cells (3-5%) + Positive + Negative control into tubes.
   *Choose pos/neg control for each antiserum from Screening Cells.
4. Incubate at RT for 5 mins.
5. Spin all tests at P5 in bench centrifuge
6. Read + record results.