RNA and DNA Seq Technologies Flashcards by Nick Tully

Q

454 Amplification?

A

Yes, Emulsion PCR

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Q

454 Sequencing by Synthesis?

A

Yes

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Q

454 Enzyme?

A

DNA polymerase

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Q

454 Detection?

A

Light from luciferase

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Q

454 Read Length?

A

Short

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Q

Ion torrent Amplification?

A

Yes, emulsion PCR

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Q

Ion Torrent Sequence By Synthesis?

A

Yes

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Q

Ion Torrent Enzyme?

A

DNA polymerase

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Q

Ion Torrent Detection?

A

H+ Semiconductor

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Q

Ion Torrent Read Length?

A

Short

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Q

Illumina Amplification?

A

Yes, Bridge

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Q

Illumina Sequence by Synthesis?

A

Yes

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Q

Illumina Enzyme?

A

DNA Polymerase

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Q

Illumina Detection?

A

Fluorescent

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Q

Illumina Read Length?

A

Short

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Q

SMRT Amplification?

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A

No

Q

SMRT Sequence by Synthesis?

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A

Yes

Q

SMRT Enzyme?

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A

xDNA polymerase

Q

SMRT Detection?

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A

Fluorescent Zerowave

Q

SMRT Read Length?

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A

Long

Q

Oxford Nanopore Amplification?

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A

No

Q

Oxford Nanopore Sequence by Synthesis?

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A

No

Q

Oxford Nanopore Enzyme?

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A

Helicase

Q

Oxford Nanopore Read Length?

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A

Long