RNAse E

Question 1

RNAse E is composed of

Answer 1

Adaptor
Regulator
rHLb 
Enolase
PNP
And RNAseE

Question 2

Draw out RNAse E

Answer 2

Do it dumb Bitch

Question 3

RNAse E mediates

Answer 3

The interplay between translation and Transcription

Question 4

What is RNase E conserved in

Answer 4

M.tb, vibrio cholera, E.coli, streptomyces

Question 5

How does RNAse E work in E. coli

Answer 5

Gets past stem loop structures (terminators) do this by making internal cuts.

For targets for exonuclease meaning more degradation

Question 6

RNAse E NTH

Answer 6

Forms stable Terrance with RNA grabbing domain

Contains 5’ sensing pocket that senses monophosphorlated end which lock terminal
Phosphate in place

Also contains Rpph which decals 5-triphosphate to monophosphate

Question 7

How does 5’end dependent pathway work

Answer 7

Minimises clashes between 5’-3’ ribosome and 3-5 exonucleases

Question 8

What is functional coupling

Answer 8

RNAse e couples with PNPase ensuring rapid degradation thus more efficiency

And rapid removal of translation initiation signals

RNAse E binds to 5’end so does not block ribosome and induces wave of 5-3 cleavage

Question 9

tRNA processing

Answer 9

One of main activists of RNAse E

Recognises ssRNA footholds on stem loop structures simultaneously

Requires multiple contacts to mediate pathway of direct entry

Doesn’t require monophosphorylated end

Initial cleavage can be by direct entry

May be RNAse E major pathway

Question 10

What is CsrA

Answer 10

A protein that binds 5’ UTR and prevents RNAse E activity

Stops interaction with ssRNA regions

Question 11

RNAse E and Ribosomes

Answer 11

RNAse E binds to 5’UTR(and TIR translation initiation region) of transcripts
Competes with ribosome binding at translation initiation

Question 12

Why is enolase part of degradosome

Answer 12

Can sense nutritional status of entire cell

Controls rate of degrading

As RNAse E may be mechanism for determine mRNA life time globally