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Flashcards in RNAse E Deck (12):
1

RNAse E is composed of

Adaptor
Regulator
rHLb
Enolase
PNP
And RNAseE

2

Draw out RNAse E

Do it dumb Bitch

3

RNAse E mediates

The interplay between translation and Transcription

4

What is RNase E conserved in

M.tb, vibrio cholera, E.coli, streptomyces

5

How does RNAse E work in E. coli

Gets past stem loop structures (terminators) do this by making internal cuts.

For targets for exonuclease meaning more degradation

6

RNAse E NTH

Forms stable Terrance with RNA grabbing domain

Contains 5' sensing pocket that senses monophosphorlated end which lock terminal
Phosphate in place

Also contains Rpph which decals 5-triphosphate to monophosphate

7

How does 5'end dependent pathway work

Minimises clashes between 5'-3' ribosome and 3-5 exonucleases

8

What is functional coupling

RNAse e couples with PNPase ensuring rapid degradation thus more efficiency

And rapid removal of translation initiation signals

RNAse E binds to 5'end so does not block ribosome and induces wave of 5-3 cleavage

9

tRNA processing

One of main activists of RNAse E

Recognises ssRNA footholds on stem loop structures simultaneously

Requires multiple contacts to mediate pathway of direct entry

Doesn't require monophosphorylated end

Initial cleavage can be by direct entry

May be RNAse E major pathway

10

What is CsrA

A protein that binds 5' UTR and prevents RNAse E activity

Stops interaction with ssRNA regions

11

RNAse E and Ribosomes

RNAse E binds to 5'UTR(and TIR translation initiation region) of transcripts
Competes with ribosome binding at translation initiation

12

Why is enolase part of degradosome

Can sense nutritional status of entire cell

Controls rate of degrading

As RNAse E may be mechanism for determine mRNA life time globally