RNAse E Flashcards
RNAse E is composed of
Adaptor Regulator rHLb Enolase PNP And RNAseE
Draw out RNAse E
Do it dumb Bitch
RNAse E mediates
The interplay between translation and Transcription
What is RNase E conserved in
M.tb, vibrio cholera, E.coli, streptomyces
How does RNAse E work in E. coli
Gets past stem loop structures (terminators) do this by making internal cuts.
For targets for exonuclease meaning more degradation
RNAse E NTH
Forms stable Terrance with RNA grabbing domain
Contains 5’ sensing pocket that senses monophosphorlated end which lock terminal
Phosphate in place
Also contains Rpph which decals 5-triphosphate to monophosphate
How does 5’end dependent pathway work
Minimises clashes between 5’-3’ ribosome and 3-5 exonucleases
What is functional coupling
RNAse e couples with PNPase ensuring rapid degradation thus more efficiency
And rapid removal of translation initiation signals
RNAse E binds to 5’end so does not block ribosome and induces wave of 5-3 cleavage
tRNA processing
One of main activists of RNAse E
Recognises ssRNA footholds on stem loop structures simultaneously
Requires multiple contacts to mediate pathway of direct entry
Doesn’t require monophosphorylated end
Initial cleavage can be by direct entry
May be RNAse E major pathway
What is CsrA
A protein that binds 5’ UTR and prevents RNAse E activity
Stops interaction with ssRNA regions
RNAse E and Ribosomes
RNAse E binds to 5’UTR(and TIR translation initiation region) of transcripts
Competes with ribosome binding at translation initiation
Why is enolase part of degradosome
Can sense nutritional status of entire cell
Controls rate of degrading
As RNAse E may be mechanism for determine mRNA life time globally
