routine hematology procedures Flashcards
(30 cards)
routine hema procedure that is not part of CBC
a. hbg
b. plt count
c. rbc count
plt count
reagents of cyanmethemoglobin that decrease amount of turbidity resulting from abnormal proteins and improve rbc lysis
a. KCN
b. potassium ferricyanide
c. KH2PO4
d. non ionic detergent
nonionic detergent
reagents of cyanmethemoglobin that replaced the Sodium Bicarbonate
a. KCN
b. potassium ferricyanide
c. KH2PO4
d. non ionic detergent
KH2PO4
reagents of cyanmethemoglobin that shortens the reaction time instead of 15 mins to 3mins
a. KCN
b. potassium ferricyanide
c. KH2PO4
d. non ionic detergent
KH2PO4
methemoglobin + potassium cyanide =
a. cyanmethemoglobin
b. sulfhemoglobin
b. methemoglobin
cyanmethemoglobin
what is the remedy for Lipemic sample
a. centrifuge the mixture and use the supernatant
b. add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank
c. dilute mixture 1:1 with H2O then multiply the results to 2
Lipemic sample
b. add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank
what is the remedy for HgB S and HgB C
a. centrifuge the mixture and use the supernatant
b. add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank
c. dilute mixture 1:1 with H2O then multiply the results to 2
hgb s ang hgb c
c. dilute mixture 1:1 with H2O then multiply the results to 2
high wbc and plt count ———–>centrifuge the mixture and use the supernatant
lipemic sample ———> add 0.01ml of patient plasma to 5.0ml of HiCn reagent and use this as a blank
how many oxygen does 1g of hbg have?
a. 1.34mL
b. 1.45 mL
c. 1.24mL
1.34mL
how many Fe++ does 100g of hgb have?
a. 0.345g
b. 1.34 ml
c. 3.37 mg
0.345g of Fe++ = 100g of Hgb
1g hgb = 3.47mg iron
1g hgb = 1.34ml of oxygen
method o fcyanmethemoglobin that uses in bloodbank to screen the blood donors
a. blood oxygen capacity
b. colorimetric method
c. copper sulfate method
copper sulfate method
what is the specific gravity of the copper sulfate solution
a. 1.053
b. 1.009
c. 1.043
1.053
van slyke method
a. blood oxygen capacity
b. blood iron content
c. copper sulfate method
blood oxygen capacity
gasometric/ vam slyke method
it uses VAN SLYKE - NEIL APPARATUS or the NATELSON MICRO GASOMETER
measures the functional hemoglobin only
a. blood oxygen capacity
b. blood iron content
c. copper sulfate method
blood oxygen content
what is the reagent used in ACID HEMATIN
a, 0.1 N HCL
b. 0.1 N NaOH
0.1 N HCL
what is the reagent used in alkali hematin
a. 0.1 N HCL
b. 0.1 N NaOH
0.1 N NaOH
sahli’s method
a. copper sulfate method
b. acid hematin
c. alkali hematin
acid hematin
Gravimetric method
a. copper sulfate method
b. blood oxygen content
c. acid hematin
copper sulfate method
abnormla hgb pigments mostly acquired due to exposure to certain drugs and chemicals
a. carboxyhgb
b. methemoglobin
c. sulfhahgb
methemoglobin
it is abnormal pigments of hemoglobin that is thought to be formed by the action of sulfonamides and aromatic amines
a. carboxyhgb
b, methemoglbn
c. sulfhahgb
sulfahgb
test to detect the Carboxyhgb
a. copper sulfate method
b. sahli’s mthod
c. katayama’s test
katayama’s test
- it uses AMMONIUM SULFIDE as a reagent and will detect as little as 10% of carboxyhgb conc
(+) carboxyhgb = ROSE RED
DIAPHORASE DEFICIENY
a. inherited methemoglbn
b. acquired methemoglbn
inherited methemoglobin
most commonly attributed to NADPH METHEMOGLBN REDUCTASE DEFICIENCY, also called DIAPHORASE DEFICIENCY
EXPOSURE TO CERTAIN DRUGS AND CHEMICALS
A. Inherited methemoglbn
b. acquired methemoglbn
ACQUIRED METHEMOGLBN
ANTICOAGULANT USED IN METHEMOGLOBIN
a. sps
b. heparin
c. edta
edta or heparin
it is abnormal pigment of hgb that is associated with severe constipation and in bacteremia with Clostridium welchii
a. carboxyhgb
b. methemoglbn
c. sulfhahgb
sulfahgbn
