RP2-calculating mitotic index

Question 1

where in plants can cells be found undergoing mitosis

Answer 1

-in meristem cells at the shoots and roots

Question 2

what is the mitotic index

Answer 2

-the ratio of cells undergoing mitosis to the total number of cells in a sample

Question 3

outline the procedure to prepare a root tip slide

Answer 3

-warm 1M of HCl at 60C in water bath
-cut root tip with scalpel and add to HCl
-leave for five mins
-remove from HCl and rinse with distilled water
-cut tip of the root tip and add to slide
-add a few drops of stain to make chromosomes visible

Question 4

formula for mitotic index

Answer 4

number of cells undergoing mitosis / number of cells in sample

Question 5

hazards and precautions for preparing a root tip slide

Answer 5

-HCl is corrosive avoid skin contact , goggles
-Toluidine blue O stain, irritant, “
-scalpel, lacerations, keep away from fingers