RP6-aseptic techniques

Question 1

state 6 aseptic techniques

Answer 1

(WBFFCK)
-wipe down all surfaces before and after experiment with antibacterial cleaner
-use a Bunsen burner in work space so convection current draws microbes away from culture (inoculating loop)
-flame the neck of the McCartney bottle to prevent bacteria entering vessel
-flame hoop before using it to transfer bacteria
-close windows to prevent air currents
-keep all vessels containing bacteria open for a minimum amount of time possible

Question 2

why is the bacteria cultured at 25C

Answer 2

-prevent growth of harmful bacteria that occur at higher temps

Question 3

How can you compare the effectiveness of different antibiotics applied to the same bacteria

Answer 3

-measure the diameter and calculate the area of the zone of inhibition on the agar

Question 4

hazards and precautions of the aseptic techniques practical

Answer 4

-naked flame, burns, handle with care or run under cold water for 20mins
-bacteria, biohazard, use disinfectant and wash hands
-disinfectant, flammable, keep away from naked flames

Question 5

why should the lid not be completely taped to the petri dish

Answer 5

-allows oxygen to enter the petri dish and prevents growth of harmful anaerobic bacteria