Safety Flashcards

1
Q

Describe behaviors that are forbidden in the lab.

A

Horseplay, pranks, practical jokes.
Eating, drinking.
Applying makeup.

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2
Q

Describe eye protection requirements.

A

ANSI Z87.1 standard
Chemical and impact resistance standard
Side shields required
Regular glasses / sunglasses are NOT a substitute
Contact lenses are NOT allowed

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3
Q

Describe what not to wear in lab.

A

Finger and wrist accessories, watch, open toed or uncovered heel shoes, long hair.

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4
Q

Name the protective equipment required when handling specimens.

A

gloves, goggles, apron

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5
Q

Describe how you are expected to treat your bench area.

A

Clean the bench before putting anything on it (you don’t know what was on it before you were there)
Keep bags under benches or with your other personal belongings by the lab entrance
Tidy bench / materials
Clean bench before leaving

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6
Q

Explain why hand washing is important.

A

Your hands may come in contact with hazardous materials.
Your hands may contaminate lab experiments.

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7
Q

When should you wash your hands?

A

Prior to a lab exercise.
After a lab exercise.
Any time your hands come in contact with potential hazards.

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8
Q

What are the important parts of effective handwashing.

A

The specific order and timing of required steps.

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9
Q

What are the steps for the lab handwashing procedure?

A
  1. Remove items from wrists and fingers.
  2. Run warm water.
  3. Wet hands.
  4. Apply soap, create lather.
  5. Clean nails.
  6. Scrub 20 seconds (front, back, and inbetween).
  7. Rinse.
  8. Dry with clean paper towel.
  9. Turn off water with paper towel.
  10. Discard paper towel.
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10
Q

What are Safety Data Sheets?

A

These specify the correct handling and disposal procedure for a substance.

also called Material Safety Data Sheets (MSDS)

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11
Q

How to properly dispose of:
Broken glass, needles, and used glass pipettes

A

These items should be disposed of in a sharps container.

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12
Q

How to properly dispose of:
Disposable items such as agar plates

A

These items should be disposed of in biohazard bins.

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13
Q

How to properly dispose of:
Paper towels used on bench area.

A

These can be disposed of in a regular trash can.

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14
Q

How to properly dispose of:
Bacterial cultures tubes

A

These should be recycled and sterilized
using an autoclave.

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15
Q

How to properly dispose of:
Chemical waste from staining bacteria

A

These should be disposed of as chemical waste as per their MSDS.

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16
Q

Where in the lab may you store food and beverage.

A

Trick question. Food and beverages may not even be brought into the lab.

17
Q

Briefly describe safety recommendations for your eyes and mouth.

A

Never touch around your eyes and mouth,
do not adjust your contacts,
do not put anything in your mouth.

18
Q

In the event of all emergencies, what should you do right away?

A

Notify your instructor!

19
Q

Briefly describe when goggles are required.

A

When working with:
UV light,
aerosols,
liquids that may splash.

20
Q

What is done after use of:
reusable items that do not require sterilization.

A

Washed in the sink.