SAS 5

Question 1

Is now considered by most laboratories to be the most conclusive method for making a definitive identification of dimorphic fungi

Answer 1

Exoantigen test:

Question 2

Serologic confirmation for systemic fungi

Answer 2

Exoantigen test:

Question 3

Also known as “Darling’s Disease

Answer 3

Histoplasmosis

Question 4

Acquired through inhalation from bird droppings

Answer 4

Histoplasmosis

Question 5

Histoplasmosis Etiologic agent:

Answer 5

Histoplasma capsulatum
Emmonsiella capsulata

Question 6

Histoplasmosis Etiologic agent:

Smallest yeast cells

Answer 6

Histoplasma capsulatum

Question 7

Histoplasmosis Etiologic agent:

Perfect - sexual stage

Answer 7

Emmonsiella capsulata

Question 8

MICROSCOPIC FEATURE FROM TISSUE FORM:
• Young 2-5 um small; oval to spherical budding cells often seen inside mononuclear cells (phagocytic cells)
• “INTRACELLULAR YEAST CELLS”

Answer 8

Histoplasmosis

Question 9

Histoplasmosis
CONFIRMATORY TEST FOR IDENTIFICATION:

Answer 9

Exoantigen Test:
• (+) with H, M, and N bands

Question 10

Causes African Histoplasmosis

Answer 10

Histoplasma capsulatum var. duboisii

Question 11

Characteristic microscopic appearance:
- Tuberculate Macroaleuriospores and Microaleuriospores

Answer 11

Histoplasma capsulatum var. duboisii

Question 12

Also known as:
• Desert fever
• Valley fever
• Desert Rheumatism
• The Bumps
• San Joaquin Valley Fever
• Posadas Werneckii

Answer 12

Coccidiodomycosis

Question 13

Acquired through inhalation of arthrospores

Answer 13

CoccidiodomycosIs

Question 14

CoccidioidomycosIs Etiologic agent:

Answer 14

Coccidiodes immitis

Question 15

Major biohazard to health

Answer 15

Coccidiodes immitis

Question 16

COLONY CHARACTERISTICS:
• At 30°C (Culture)
- Colonies are usually fluffy white but may be pigmented gray, orange, brown or yellow
- Mycelium is adherent to the agar surface in some portions of the colony “cob-web” like growth

Answer 16

Coccidiodomycosis

Question 17

MICROSCOPIC FEATURES FROM CULTURE:
• Chains of alternate BARREL-SHAPED ARTHROCONIDIA
• Hyphae are small and often arranged in rope-like strands and racquet forms are seen in young cultures

Answer 17

CoccidiodomycosIs

Question 18

MICROSCOPIC FEATURES FROM TISSUE FORM:
• Round SPHERULES 30-60 um in diameter containing 2-5 um
ENDOSPORES

Answer 18

Coccidiodomycosis

Question 19

Coccidiodomycosis
• Confirmatory Test for ID:

Answer 19

Exoantigen test:
• (+) for Ill, F and TP bands

Question 20

Diagnostic procedure for detecting active infection with
C. immitis:

Answer 20

A. Coccidioidin skin test
B. Serology: Compliment fixation test

Question 21

Also known as:
• Gilchrist’s Disease
• Chicago Disease
• North American Blastomycosis

Answer 21

Blastomycosis

Question 22

Chronic granulomatous disease of skin, mucous membrane, lymph nodes and internal organs

Answer 22

Blastomycosis

Question 23

Blastomycosis Etiologic agent:

Answer 23

Blastomyces dermatitidis, Ajellomyces dermatitidis

Question 24

Blastomycosis Etiologic agent:

(perfect-sexual stage)

Answer 24

Ajellomyces dermatitidis

Question 25

Clinical characteristics of the cutaneous skin lesion:
- Very rough and crusty
- Somewhat elevated
- Well marginated

Answer 25

Blastomycosis

Question 26

COLONY CHARACTERISTICS:
• At 30°C (Culture);
- Colonies are white to cream to tan, some with drops of exudates present, fluffy to glabrous and adherent to the surface with tufts of hyphae projecting upwards (“PRICKY STATE”) on medium with blood

Answer 26

Blastomycosis

Question 27

MICROSCOPIC FEATURES FROM CULTURES:

• Hyphae 1-2um in dm are present, single pyriform conidia are produced on short to long conidiophores (“LOLLIPOP conidia”)

Answer 27

Blastomycosis

Question 28

MICROSCOPIC FEATURES FROM TISSUE FORM:
• 8-15 um BROAD-BASED BUDDING thick walled yeast cells

Answer 28

Blastomycosis

Question 29

Confirmatory test or ID:
- Broad-based budding cells
- Exoantigen test (+ with A band)

Answer 29

Blastomycosis

Question 30

Also known as:
• South American Blastomycosis
• “Para” or “Paracocci”
• Almeida’s Disease
• Lutz Splendore Almeida’s Disease

Answer 30

Paracoccidioidomycosis

Question 31

Paracoccidioidomycosis Etiologic agent:

Answer 31

Paracoccidioides brasiliensis

Question 32

MICROSCOPIC FEATURES FROM TISSUE FORM:
• Multiple budding cells (resembling a “MARINER’S WHELL”)
maybe present

Answer 32

Paracoccidioidomycosis

Question 33

Confirmatory Test for ID of Paracoccidioidomycosis

Answer 33

• Exoantigen test:
  • (+) for 1,2 and 3
• Serologic test:
  • CF