SEC HEMO TESTS Flashcards
(34 cards)
Measures the period required for free formation of
blood to clot after it has been removed from the body
Clotting Time
Clotting time 2 methods
• Methods:
o Capillary Blood Method
▪ Slide drop method
▪ Capillary method (Dale and Laidlaw’s)
o Whole Blood (Lee and White Method)
• Methods:
o Capillary Blood Method
▪ Slide drop method
□ Normal value:
• Methods:
o Capillary Blood Method
▪ Slide drop method
□ Normal value: 2-4 mins
▪ Capillary method (Dale and Laidlaw’s)
o Whole Blood (Lee and White Method)
▪ Normal value:
7-15mins
Monitors coagulation factors of the extrinsic and
common pathways
proth time
Used to monitor oral anticoagulant therapy
proth time
When______ is added to PPP along
with calcium, it reacts with factor VIIa to convert
factor X to Xa.
thromboplastin
Factor Xa, along with factor Va, phospholipids,
and calcium, converts prothrombin to____.
thrombin
o Standardized way of reporting PT results
o Introduced to minimize the difference in PT
results due to different reagent-instrument
combinations
INR - International Normalized Ratio
Used to monitor heparin therapy (heparin -
antithrombin)
Activated Partial Thromboplastin Time (APTT)
Stypven Time
aka?
Russell’s Viper Venom Time
Reagent: of Stypven Time
East Indian Viper venom (Vipera russelli)
Normal value Stypven time
6-10 secs
Reptilase - thrombin-like enzyme from the reptile
which Converts fibrinogen to fibrin
Bothrops atrox
Detects fibrinogen deficiency
• Unaffected by heparin therapy
Reptilase Time
Reptilase differs from thrombin in which
reptilase cleaves ______, while
thrombin cleaves fibrinopeptides A and B
fibrinopeptide A
Duckert’s Test
aka?
• Detects Factor XIII deficiency
• Aka 5M Urea Solubility Test
Duckert’s Test reagent
1% monochloroacetic acid
Principle of test; Patient’s plasma is clotted by the addition of
calcium chloride.
Duckert’s Test
Factor XIII deficiency: clot is soluble to _____
5M urea
Presence of Factor XIII: clot is insoluble to 5M urea for
____ hrs
24 hrs
Mixing Studies
• Aka??
• Aka substitution studies
A procedure done to identify specific factor
deficiencies by mixing correction reagents with a
patient’s plasma, and performing PT and APTT
mixing studies
• Screening procedure for the measurement of
fibrinolytic activity
Euglobulin Clot Lysis Time
