Serological

Question 1

What is RPR?

Answer 1

Non treponemal test for serological testing of antibody reagin

Question 2

What is reagin?

Answer 2

Antibody produced in response to an infection of syphilis caused by treponema pallidum

Question 3

What part of blood is reagin found in?

Answer 3

Serum/plasma

Question 4

Carbon charcoal is what kind of particle that allows us to see reagin macroscopically?

Answer 4

Vehicle

Question 5

RPR is what kind of test?

Answer 5

Screening test

Question 6

Does RPR require confirmation?

Answer 6

Yes

Question 7

RPR is reactive if what occurs?

Answer 7

Flocculation

Question 8

RPR is non reactive if what occurs?

Answer 8

No flocculation

Question 9

What is the confirmatory test for RPR?

Answer 9

Fluorescent treponemal antibody-absorbed test (FTA-ABS)

Question 10

What is on the slide during FTA-ABS

Answer 10

Dead t. pallidum and patient’s serum

Question 11

What is added to the slide during FTA-ABS to detect antibody?

Answer 11

Fluorescein-labeled anti-human globulin

Question 12

Positive FTA-ABS will look like what?

Answer 12

Fluorescent

Question 13

Negative FTA-ABS will look like?

Answer 13

Non-Fluorescent

Question 14

What tube should you collect blood in for RPR testing if using serum?

Answer 14

Red/SST

Question 15

What tube should you collect blood in for RPR if testing plasma?

Answer 15

Purple

Question 16

After centrifugation, how should serum look?

Answer 16

Clear and non-hemolyzed

Question 17

What kind of needle is used for RPR test kit?

Answer 17

20g blunt tip

Question 18

How should you mix antigen suspension in RPR test kit?

Answer 18

Shake vigorously for 10-15seconds

Question 19

How much of patient’s specimen should be used for RPR test kit?

Answer 19

1 drop

Question 20

How long should specimen stay on rotator for RPR test?

Answer 20

8 minutes at 100 rpm

Question 21

When should you read results of RPR test?

Answer 21

At 8 minutes

Question 22

How should you read the RPR test kit?

Answer 22

Rotate and tilt kit by hand 3-4 times

Question 23

Is weak-reactive a result for RPR testing?

Answer 23

No, only for QC

Question 24

Antigen needle is calibrated to what in RPR test kit?

Answer 24

60 drops per 1 ml

Question 25

What causes infectious mono nucleosis?

Answer 25

Epstein-barr virus

Question 26

What are mono sx?

Answer 26

Fever, malaise, lethargy, sore throat w/ exudate, enlarged lymph nodes on the neck, mild hepatitis, enlarged spleen and occasional blotchy skin rash

Question 27

Mono causes increased numbers of abnormal lymphocytes and monocytes cause which antibody to form?

Answer 27

Heterophile antibody

Question 28

What part of blood is used for monospot testing?

Answer 28

Serum or plasma

Question 29

When should you test the blood collected for mono spot testing?

Answer 29

Within 24 hours if stored between 2-8C

Question 30

What temperature should latex reagent and controls be for monospot testing?

Answer 30

Room temp 20-30C

Question 31

How should mixing of the latex reagent be conducted for mono spot testing?

Answer 31

Gently shake

Question 32

How much specimen should be used for monospot testing?

Answer 32

1 drop

Question 33

How much latex reagent should be mixed with sample during monospot testing?

Answer 33

1 drop

Question 34

What do you use to mix drops during monospot testing?

Answer 34

Stirrer ensuring coverage of entire surface

Question 35

How long should monospot slide be rotated and if on a rotary shaker, at what setting?

Answer 35

3 minutes, shaker should be at 60-100 rpm

Question 36

Positive monospot shows?

Answer 36

Agglutination

Question 37

Negative monospot shows?

Answer 37

Smooth, homogenous solution, no agglutination