Session 2 Flashcards
(62 cards)
Each colony seen on an agar plate represents…
A single bacteria
In theory what is more effective, alcohol hand gel or hand washing?
Alcohol hand gel
Adenovirus is a common viral infection, what is the best way to specially diagnose adenovirus?
Viral PCR
Culturing a virus is possible but it has to be done with…
How is the virus viewed?
Live cells
Electron microscope
What are the two main groups of toxins that arise from bacteria and cause damage?
Endotoxins
Exotoxins
What is an endotoxin?
A toxin that causes harm and is a part of the bacteria structure
What is the best example of an endotoxin?
Outer membrane of gram negative bacteria (lipopolysaccharide)
What are the properties of the lipopolysaccharide? What can it cause in the human body?
It is highly toxic
Can cause a major inflammatory response
Is sepsis is usually associated with gram positive or gram negative bacteria? Why?
Gram negative
Due to the highly toxic lipopolysaccharide in gram negatives which can trigger a major inflammatory response
What colour do gram negative bacteria stain in a gram stain? Why?
Red/Pink
Due to the presence of LPS/outer membrane
What colour do gram positive bacteria stain in a gram stain?
Purple
Rank the 4 main types of microorganisms that cause disease in humans from smallest to largest…
Virus
Bacteria
Fungi
Parasites
Viruses can only be viewed using which type of microscope?
Electron microscope
Can bacteria be viewed under a light microscope?
Yes
What are prions? Give an example of a disease caused by prions?
Proteins that generate copies of themselves and can spread from person to person and cause disease
Mad Cow Disease
Why are prions particularly dangerous and able to be transmitted via surgical equipment?
They are not destroyed in the high pressure/high temperature sterilisation of surgical instruments
Are bacteria eukaryotic or prokaryotic?
Prokaryotic
How are viruses commonly classified?
By the nucleic acids they possess (e.g. RNA or DNA, single stranded or double stranded)
Are viruses enveloped?
Can exist with or without an envelope
Viruses often show tissue tropism, what is meant by this?
They prefer particular cell lines
What are the different layers of the bacteria cell membrane (from inside to out)?
Plasma membrane
Periplasmic space
Petidoglycan (cell wall)
(Periplasmic space + LPS - in gram negative bacteria)
What are exotoxins?
Proteins secreted by the bacteria and go out into the surrounding blood and tissue and damage tissues
Exotoxins that act on the bowel are called…
Exotoxins that act on nerves are called…
Enterotoxin
Neurotoxin
What are the different layers of a virus (from inside to outside)?
Nucleic acid (DNA/RNA, SS or DS)
Protein Coat
(Envelope - some virus)
Spikes