session 3-cell ultrastructure and organelles Flashcards

1
Q

what is the limit of resolution for an electron microscope?

A

-0.002nm

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2
Q

what is an endosome, peroxisome and cytosol?

A
  • endosome=membrane bound. assists in transport pathway from plasma membrane to lysosome
  • peroxisome=small organelle in cytoplasm which contains catalase and oxidases
  • cytosol=aqueous part of cytoplasm
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3
Q

in terms of the phospholipid bilayer, what does amphipathic mean?

A

having both hydrophilic and phobic parts

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4
Q

what is the limit of resolution for light microscopes?

A

-0.2um

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5
Q

what is the glycocalyx?

A

cell ‘coat’ made up of oligosaccharides (a couple of monosaccharides) and polysaccharide side chains on outside of plasma membrane-side chains give cell SPECIFICITY

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6
Q

give 3 functions of the plasma membrane (plasmalemma)

A
  • selective permeability
  • exo/endocytosis
  • signalling
  • transport of materials
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7
Q

what is the role of the nucleus?

A
  • stored cells DNA
  • CO ORDINATES cell activities-metabolism,mitosis, protein synthesis
  • contains chromatin
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8
Q

what is chromatin?

A

-unstructured DNA that will organise to form chromosomes in mitosis

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9
Q

what is the nuclear envelope attached to?

A

-a network of tubules (AKA endoplasmic reticulum)

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10
Q

what does the nucleolus synthesise?

A

ribosomes

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11
Q

name 3 places the smooth ER is found

A

liver, mammary glands, ovaries/testes

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12
Q

when vesicles bud off from the RER to be moved to the golgi, what face do they attach to?

A

-cis face of golgi (convex side) then to concave (trans) side

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13
Q

what is the function of the golgi?

A

-modify, package,sort and concentrate proteins

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14
Q

what happens to some vesicles?

A

become lysosomes

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15
Q

where are lysosomes generated?

A

golgi

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16
Q

what do you call lysosomes that have digested their contents but contain indigestible remnants?

A

residual bodies

17
Q

what is produced in peroxisomes?

18
Q

what enzyme uses h2o2 to oxidise things such as phenols, formaldehyde and alcohol?

19
Q

what are the 2 main substrates of ATP production in the mitochondria?

A

glucose and fatty acids

20
Q

which 2 places would you find many mitochondria?

A
  • liver

- skeletal muscle cells

21
Q

in terms of mitochondria, what does female lineage mean?

A

-inheriting all mitochondria from mother

22
Q

name the 3 types of cytoskeleton (maintains or changes cell shape)

A

-microfilaments, microtubules and intermediate filaments

23
Q

give 3 roles of the cytoskeleton

A
  • supports plasma membrane and organelles
  • helps constituents move
  • helps muscles contract
24
Q

what is the diameter of microfilaments?

25
what are microfilaments made up of?
-2 strings of actin twisted together (associated with ATP)
26
what is the diameter of intermediate filaments?
10nm
27
where would you find intermediate filaments?
nerves and some epithelia-form tough network
28
what are microtubules made up of?
-13 alpha and beta subunits form wall of hollow microtubules
29
where are microtubules found?
-near structures that move e.g. nerve fibres, mitotic spindle, cores of cilia and flagella
30
Can mitochondria divide on their own because they have their own genetic info?
Yes
31
What is the role of the nucleolus?
Make rRNA