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Molec Lab > Sir Gerard Ghani Lec > Flashcards

Sir Gerard Ghani Lec Flashcards

1
Q

Practices implemented to prevent accidental/unintentional release/exposure to pathogens

A

Biosafety

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2
Q

Designed to prevent loss, theft, misuse, diversion or intentional release of pathogens

A

Biosecurity

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3
Q

Protecting people from bad bugs

A

Biosafety

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4
Q

Protecting bad bugs from bad people

A

Biosecurity

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5
Q

unauthorized access in the laboratory

A

Biosecurity

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6
Q

Recapping of needles

A

Biosafety

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7
Q

Wearing of PPE

A

Biosafety

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8
Q

Work overload

A

Neither

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9
Q

Staff with huge debt

A

Neither

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10
Q

Laboratory acquired infections

A

Biosafety

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11
Q

Leakage of lab data

A

Biosecurity

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12
Q

Lab technologist is a member of terrorist group

A

Biosecurity

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13
Q

Lack of training

A

Neither

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14
Q

Disgruntled staff

A

Biosecurity

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15
Q

effective management of risks posed by working with infectious agents and toxins in laboratories. Its goal is to identify and reduce risk before they evolve into neear incidents

A

Biorisk management

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16
Q

3 components of Biorisk management

A

Assessment, Mitigation, and Performance

17
Q

Enumerate the steps in Donning of PPE (in order)

A

Hand hygiene-Shoe cover-Hand hygiene-Inner gown-inner gloves-N95 mask-head cap-outer gown-face shield-outer gloves

18
Q

Enumerate the steps in Doffing of PPE (in order)

A

Outer gloves-outer gown-shoe cover-face shield-head cap-inner gloves-inner gown-hand hygiene-N95 mask-hand hygiene

19
Q

This equipment is used for preparing Mastermix

A

PCR Cabinet (Reagent preparation room)

20
Q

It is used for RNA Extraction and Template Addition

A

Biosafety cabinet class II (Extraction room)

21
Q

used for the treatment of biohazardous wastes

A

Autoclave (Doffing area)

22
Q

What is the principle of autoclave?

A

Steam under pressure

23
Q

Thermal cycler is also termed as _______room

A

Amplification room

24
Q

2 types of waste generated by molecular laboratory

A

General waste and Biohazardous waste

25
Q

TRUE OR FALSE

DNA and RNA are macromolecules comprised of many nucleotides

A

TRUE

26
Q

3 parts of a nucleotide

A

A nitrogenous base, 5 carbon sugar, and 1 Phosphate group

27
Q

What enzymes unwind the DNA?

A

DNA helicase

28
Q

This enzyme is for the synthesis of short RNA primers

A

RNA primase

29
Q

adds nucleotides to the primers and extends the strand

A

DNA polymerase III

30
Q

degrades the RNA primer and replaces it with DNA

A

DNA Polymerase I

31
Q

connect two strands of RNA

A

DNA ligase

32
Q

Synthesis of RNA (mRNA) through DNA by RNA polymerase

A

Transcription

33
Q

Synthesis of polypeptide/protein through mRNA

A

Translation

34
Q

Where does translation happen?

A

in the ribosomes

Molec Lab (6 decks)

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