Slides And Photomicrographs

Question 1

What can an optical microscope be used to observe? (3)

Answer 1

Living organisms (amoeba)
Smear preparations of human blood and cheek cells
Thing sections of animal, plant and fungal tissue

Question 2

What is light interference?

Answer 2

A way of observing a specimen without staining

Question 3

What does light interference involve?

Answer 3

Dark background against which illuminated specimens show up

Question 4

What stain is used to colour chromosomes dark red?

Answer 4

Acetic orcein

Question 5

What is used to stain the cytoplasm of a cell?

Answer 5

Eosin

Question 6

What does Sudan red stain?

Answer 6

Lipids

Question 7

Iodine in potassium iodide stains starch blue/black. What colour does it stain cellulose?

Answer 7

Yellow

Question 8

What happens to a specimen during the preparation process? (4)

Answer 8

Dehydrated
Embedded in wax
Special instrument makes small slices called sections
Stained and mounted in chemicals to preserve

Question 9

Why are specimens embedded in wax?

Answer 9

To prevent distortion

Question 10

What is the equation used to calculate size of an image under a microscope?

Answer 10

Magnification=image/actual