Sloid Phase Extraction

Question 1

What is solid phase extraction

Answer 1

separation process to
extract compounds (analyte)
from a mixture of impurities.
• SPE is use to
- concentrate and purify samples for
analysis.
- to isolate analytes of interest from a
wide variety of matrices - urine, blood,
water samples, beverages, soil, animal
tissue & consumer products.

Question 2

4 steps for solid phase extraction

Answer 2

1. Conditioning

The SPE cartridge is activated with a solvent to wet the sorbent material and remove any impurities.

2. Loading sample

The sample containing the target analyte is passed through the cartridge. The target compound binds to the sorbent, while unwanted components pass through cartridge. analytes in the sample
will interact and retain on the
sorbent while the solvent, salts,
and other impurities pass
through the cartridge.

3. Rinsing

The cartridge is washed with a suitable solvent to remove any remaining impurities that might interfere with the analysis.

4. Eluting

The target analyte is eluted from the cartridge using a stronger solvent specific to its properties. This concentrated eluate is then collected for further analysis.
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Question 3

separation ability of SPE

Answer 3

preferential affinity of desired
or undesired solutes in mobile
phase (liquid) for a stationary
phase (solid) through which the
sample is passed.

The stationary phase is usually
packed in a syringe-shaped
cartridge, which can be mounted
on a commercially available
extraction manifold.

A typical SPE manifold can
accommodate 12 or 24 SPE
cartridges so that many samples
can be processed
simultaneously.

• Many SPE manifolds are
**equipped with a vacuum port;
application of a vacuum speeds
up the extraction process by
pulling the liquid sample
through the stationary phase.

The analyte is collected with a
sample tube rack inside the
manifold, which collects the
sample as it is eluted off the
cartridge.

• SPE cartridges are available with
a variety of different stationary
phases, each of which can
separate analytes by a different
chemical mechanism.

Most stationary phases are
based on a bonded silica
material that is derivatized with
a specific functional group.

• Some of these functional groups
include hydrocarbon chains of
variable length (for reversed
phase SPE), quaternary
ammonium or amino groups
(for anion exchange), and
sulfonic acid or carboxyl groups
(for cation exchange)