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Question 1

Relationship between viral genome size and protein coding capacity

Answer 1

The bigger the genome, the more protein it can encode

Question 2

+ sense strand

Answer 2

Same sequence as protein-coding mRNA

Question 3

Baltimore Classification

Answer 3

Classification of viruses based on how they make mRNA

Question 4

What type of forces join capsid subunits together?

Answer 4

Non covalent forces

Question 5

2 types of capsid symmetry

Answer 5

Icosahedral and helical

Question 6

What dictates the length of the TMV virion?

Answer 6

The size of genome. Longer genome, longer virion (helical)

Question 7

icosahedron specs

Answer 7

• 6-fold symmetry on each face

- 5-fold symmetry on each vertex

Question 8

What T numbers has pentons and hexons

Answer 8

T>1

Question 9

Penton

Answer 9

• groups of 5 subunits

- make up the 12 vertices

Question 10

Hexon

Answer 10

• groups of 6 subunits

- make up the faces

Question 11

When T>1, how many penton subunits are there?

Answer 11

always 60

Question 12

How to find out number of hexon subunits for T>1?

Answer 12

60T-60

Question 13

Give examples of viruses with dsDNA genomes

Answer 13

herpesvirus, bacteriophage T4

Question 14

Examples of viruses with ssDNA genomes?

Answer 14

parvovirus

Question 15

What complexes do DNA viruses need to make mRNA and duplicate genome?

Answer 15

• DNA-dependent DNAP

- DNA-dependent RNAP

Question 16

Examples of viruses with dsRNA

Answer 16

reovirus

Question 17

What complex do RNA viruses need to make mRNA and duplicate genome?

Answer 17

• RNA-dependent RNAP

Question 18

examples of + sense ssRNA

Answer 18

poliovirus, Zika virus

Question 19

examples of - sense ssRNA

Answer 19

influenza, measles

Question 20

examples of retroviruses

Answer 20

HIV

Question 21

ICTV scheme of classification

Answer 21

takes into account properties of virus particle, genetic relationships, genome structure, plus mRNA generation

Question 22

Most DIRECT method of detecting and quantifying virus particles

Answer 22

Electron microscopy (EM)

Question 23

Downfall of EM

Answer 23

Only gives the particle count and not the proportion of particles that are infectious

Question 24

INDIRECT methods of detecting and quantifying viral genome (x2)

Answer 24

• nucleic acid hybridization

- PCR

Question 25

What technique can detect viral structural proteins using specific antibodies?

Answer 25

Immuno-detection assays.

Question 26

Technique used to detect infectivity

Answer 26

Plaque assay

Question 27

Particle-to-PFU ratio

Answer 27

how many virus particles does it take to cause one plaque

Question 28

Tissue Culture Dose 50 (TCD50)

Answer 28

Dilution resulting in half the cultures receiving a single plaque

Question 29

T or F: During infection, viral infectivity disappears when the viral genome is released from capsid and does not reappear until progeny virions are assembled.

Answer 29

True

Question 30

Multiplicity of Infection (MOI)

Answer 30

How many virions are added per cell