Smiley-7: Negative and dsRNA Expression and Replication Flashcards
(19 cards)
What must -ve and dsRNA do before any protein translation?
transcribe their genome
-ve strand RNAs are divided into 2 groups:
- monopartite genome
- multipartite genome
VSV (vsicular stomatitis virus) genome description (x3)
multipartite or monopartite?
- monopartite
- -ve strand
- RNA
How many genes does VSV have in its genome?
5
What proteins comprise the RdRNAP of VSV?
L and P proteins
How does VSV create polyA tail?
stuttering mechanism
How does VSV make a full-length + strand genome?
N protein coats -ve strand genome to prevent polyadenylation and termination reactions
How is paramyxo virus’ ORF different from others?
some copies of mRNA encoding V protein are edited by adding extra G residues to change reading frame to make another mRNA encoding a different protein
How does “editing” occur in paramyxo viruses?
stuttering in a run of C’s in template strand during transcription
Describe influenza’s genome (x3)
multipartite or monopartite?
- multipartite
- ss
- -ve sense RNA
How many RNA segments are present in influenza genome?
8
Where does influenza genome go to get transcribed?
host’s nucleus
How does influenza acquire 5’ cap?
its RdRNAP cleaves 5’ cap of host cell and attaches it to the -ve sense template
How is influenza virus polyadenylated?
stuttering reaction
What mediates the switch from transcription to replication in influenza?
NP protein
Segment reassortment
high frequency genetic “recombination” system where virus cannot distinguish between homologous genome segments of 2 diff. species and recombines them together
Describe reovirus genome (is it capped?) (x3)
- dsRNA
- multipartite
- only +ve strand is capped
How many segments in reovirus genome ?
10
Where does transcription and replication occur in reoviruses?
in their partially uncoated capsid
