SNAREs 1 Flashcards
1
Q
How big is a vesicle ?
A
50 to 100 nm
2
Q
3 approaches to study machinery
A
Biochemical reconstitution
Yeast genetics
Cloning
3
Q
What is biochem recons
A
intra golgi transport assay
4
Q
What does sec 1 encode
A
SNARE binding prot
5
Q
What does sec 17 encode
A
alpha SNAP
6
Q
What does sec 18 encode
A
NSF
7
Q
What does NSF do ?
A
inhibits transport
8
Q
SNare hypo
A
Some for each transport step
provide spe
are sufficient to drive fusion
NSF and ATP catalyse fusion (wrong)
9
Q
What are R and Q snares
A
R= vesicle
Q= on m.
ALways 1:3 ratio
10
Q
What is actual role of NSF
A
Unscrews prot using ATP when cis-SNARE complex state
