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Stem Cell and Regenerative Medicine > soft tissue > Flashcards

Flashcards in soft tissue Deck (11):
1

what is mechanical mismatch ?

when you have two uncomplimntray tissues between bone implant and bone, or suture retention etc

2

how can you do tissue engineering with a regen approach?

- you can take some cells so autologous and then expand and put on a scaffold and implant back

3

what is so difficult about tissue engineering?

e are lots of things to think about, mechanical chemistry, natural a morphology,cells development, scaffolds, degradation

4

what is the structure of an alpha helix that you need to know ?

- you can make an alpha helix sheet and a beta sheet
- you need to understand that in an alpha helix there is a bond between the NO and the CO every 3.6 residues- this results in a kink
- you need hydrophobic polar polar hydrophobic polar polar polar seuqence too

5

how can you put two alpha helix together?

- make a complimentary coil with hydrbphobc residue in the middle so that they can come together and make a coiled coiled
- you make sure they are staggered so that you can make a sticky ended dimer

6

what is a sticky ended dimer?

staggered coiled coils

7

what is a self creating fibre?

- straggered complimentary helixs that will stick to each other

8

what do you stick to the end of the fibre? why

hSAF-p1 groups which you can add functional groups on and this means you can stick RGDS on these aside groups which can add stickiness

9

show was it shown that the decorated ells worked?

- they placed neural stem cells and could see that the neural stem cells were decorating the matrix well throughout the 3D cell and they produced axons maybe
- they are more likely yo be guided towards a wound site with these decorated ECM structure - can pass current through the cells

10

can the aside group be added with things other than RGDS?

yes can add sequences from BMP and VEGF etc that can influence- add a coktial

11

can you alter the stiffness?

yes and then you can use for different tisuses- floppy for brain - can modify regions to make gels behave like brain like tissue