Specialized Growth Media

Question 1

Corynebacterium diphtheriae

Answer 1

Tellurite agar, Loeffler’s medium

Question 2

Group D streptococci

Answer 2

Bile esculin agar

Question 3

Staphylococci

Answer 3

Mannitol salt agar

Question 4

N. meningitidis, N. gonorrhoeae from sterile sites (blood, CSF)

Answer 4

Chocolate agar

Question 5

N. gonorrhoeae from nonsterile sites (oral, genitourinary tract discharge)

Answer 5

Thayer-Martin agar

Question 6

Haemophilus influenzae

Answer 6

Chocolate agar + factors X and V

Question 7

Clostridium perfringens

Answer 7

Egg yolk agar

Question 8

Mycobacterium tuberculosis

Answer 8

Lowenstein-Jensen (Middlebrook) agar

Question 9

Vibrio cholerae

Answer 9

Thiosulfate Citrate Bile Salts (TCBS)

Question 10

Bordetella pertussis

Answer 10

Bordet-Gengou agar OR Regan-Lowe agar

Question 11

Legionella pneumophila

Answer 11

Charcoal-yeast extract

Question 12

Campylobacter, Helicobacter

Answer 12

Skirrows agar

Question 13

Borrelia burgdorferi

Answer 13

Barbour-Stoaner-Kelly (BSK) agar

Question 14

Mycoplasma pneumoniae

Answer 14

Eaton agar

Question 15

Pseudomonas aeruginosa

Answer 15

Cetrimide agar

Question 16

Salmonella, Shigella

Answer 16

Xylose-Lysine-Deoxycholate (XLD) agar

Question 17

Leptospira interrogans

Answer 17

Ellinghausen-McCullough-Johnson-Harris (EMJH) agar OR Fletcher’s agar

Question 18

Isolates Gram positive cocci; determines pattern of hemolysis

Answer 18

Blood agar

Question 19

Isolates enteric Gram negative rods; selects against Gram positive bacteria and differentiates between lactose fermenters and nonfermenters

Answer 19

MacConkey agar

Eosin-methylene blue (EMB) agar

Question 20

Isolates enteric Gram negative rods; distinguishes lactose fermenters from nonfermenters and H₂S producers from nonproducers

Answer 20

Triple sugar iron (TSI) agar