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Clinical Bacteriology > Specimen Management > Flashcards

Specimen Management Flashcards

(92 cards)

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1
Q

WHEN should specimen be collected?
DURING ______________
WITHIN _______________
BEFORE ______________

A

During Acute Phase
Within 2-3 Days for Viral Infection
Before Antibiotics are Administered

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2
Q

Container for Abscess, Lesions, Wounds

A

Swabs (aerobic) or sterile tube (for aspirates)

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3
Q

For Abscess, Lesions, Wounds:

Wipe around the area with _______ or _______

A

sterile saline or 70% alcohol

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4
Q

Container for Urine

A

Clean, screw cap

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5
Q

Container for Sputum

A

Clean, screw cap

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6
Q

Sputum collection procedure for deep cough only

A

Expectorated Sputum

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7
Q

Sputum collection procedure with inhale solution

A

Induced Sputum

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8
Q

Container for Stool

A

Clean, screw cap

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9
Q

Bacterial stool are preferably performed _________

A

once a day for 3 days

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10
Q

Container for Blood

A

Blood culture media set

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11
Q

For Blood Collection, disinfect site with _______ and _______

A

70% alcohol and Povidone iodine

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12
Q

Container for CSF

A

Clean, screw cap

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13
Q

Which CSF tube is used for bacteriology laboratory use?

A

Tube 2

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14
Q

Specimen label must include

A

Patient’s name
Hospital number/DOB
Date and TOC
Source

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15
Q

Ideally, collected specimen must be transported to the laboratory within ________ and not longer than ________

A

30 minutes,

2 hours

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16
Q

CSF - transport within _______ after

collection

A

15 mins

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17
Q

Anaerobic specimen - transport not longer than

_________.

A

10 mins

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18
Q

Sensitive to change in temperature

A

Neisseria meningitidis

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19
Q

Sensitive to change in PH

A

Shigella

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20
Q

Transport condition of Body fluids

A

Immediately at RT

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21
Q

Transport condition of Corneal scrapings

A

Immediately at RT

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22
Q

Transport condition of bone

A

Immediately at RT

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23
Q

Transport condition of gastric aspirate

A

Immediately at RT

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24
Q

Transport condition of prostatic samples

A

Immediately at RT

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25
Transport condition of suprapubic aspirate
Immediately at RT
26
Transport condition of tissue
Immediately at RT
27
Transport condition of gastric biopsy
Within 1 hour at RT
28
Transport condition of stool
Within 1 hour at RT
29
Transport condition of abscess, lesions, wound
Within 2 hours at RT
30
Transport condition of blood
Within 2 hours at RT
31
Transport condition of BMA
Within 2 hours at RT
32
Transport condition of genital tract specimens
Within 2 hours at RT
33
Transport condition of respiratory tract specimens
Within 2 hours at RT
34
Transport condition of urine
Within 2 hours at RT
35
Transport condition of urine samples from foley catheter
Within 2 hours at 4°C
36
Transport condition of Hair, nails, skin scrapings (Fungal | culture)
Within 24 hours at RT
37
A) Transport condition of specimens with transport medium/preservatives B) What are these specimens?
A) Within 24 hours at RT B) Stool, Rectal Swab, Urine
38
Boric acid is used for _______ to maintain quality and quantity of bacteria
Urine
39
Cary-blair media is used for
Stool
40
Anticoagulants for specimen preservation
Sodium Polyanethol Sulfate, Heparin
41
Anticoagulant that inhibits growth of gram(+) bacteria and yeast for viral cultures
Heparin
42
transport medium used for Neisseria gonorrhea suspected specimen
JEMBEC
43
Storage Condition for CSF
37°C
44
Storage Condition for Urine
4°C
45
Storage Condition for Stool
4°C
46
Storage Condition for Viral specimen
4°C
47
Storage Condition for Swabs
4°C
48
Storage Condition for Foreign Devices
4°C
49
Storage Condition for Serum (for 1 week)
-20°C
50
Storage Condition for tissues
-70°C
51
Storage Condition for specimens for long term storage
-70°C
52
Expectorated sputum with ________ and ________ in Gram staining are rejected
<25 WBCs and >10 epithelial cells
53
``` Descriptions of the Levels of Specimen Prioritization 1 - 2 - 3 - 4 - ```
1 - Critical/invasive 2 - Unpreserved 3 - Quantitation Required 4 - Preserved
54
Level of Specimen Prioritization of Amniotic Fluid
1 - Critical/invasive
55
Level of Specimen Prioritization of Blood
1 - Critical/invasive
56
Level of Specimen Prioritization of Brain
1 - Critical/invasive
57
Level of Specimen Prioritization of CSF
1 - Critical/invasive
58
Level of Specimen Prioritization of Heart Valves
1 - Critical/invasive
59
Level of Specimen Prioritization of Pericardial Fluid
1 - Critical/invasive
60
Level of Specimen Prioritization of Body Fluids
2 - Unpreserved
61
Level of Specimen Prioritization of Bone
2 - Unpreserved
62
Level of Specimen Prioritization of Drainage from Wounds
2 - Unpreserved
63
Level of Specimen Prioritization of Stool
2 - Unpreserved
64
Level of Specimen Prioritization of Sputum
2 - Unpreserved
65
Level of Specimen Prioritization of Tissue
2 - Unpreserved
66
Level of Specimen Prioritization of Catheter Tip
3 - Quantitation Required
67
Level of Specimen Prioritization of Urine
3 - Quantitation Required
68
Level of Specimen Prioritization of Tissue for quantitation
3 - Quantitation Required
69
Level of Specimen Prioritization of stool in preservative
4 - Preserved
70
Level of Specimen Prioritization of urine in preservative
4 - Preserved
71
Level of Specimen Prioritization of swabs in holding medium (aerobic and anaerobic)
4 - Preserved
72
Most cultures grow between __°C and __°C in incubation
35°C and 37°C
73
Time required in incubation
most are held for 2-3 days | some are held for 5-7 days
74
Waste Disposal Decontamination with the use of Chemical - - -
Sodium Hypochlorite (10%) Chlorine Dioxide Peracetic Acid
75
Waste Disposal Decontamination with the use of Thermal - -
Moist Heat | Dry Heat
76
process of destruction of all forms of microbial life
STERILIZATION
77
lowest temperature at which all organisms in particular liquid suspension will be killed in 10 minutes
THERMAL DEATH POINT
78
minimal length of time for all bacteria in particular liquid culture to be killed at a given temperature.
THERMAL DEATH TIME
79
time in which 90% of bacterial population will be killed at a given temperature
DECIMAL REDUCTION TIME
80
kills microorganisms by oxidation effects
Dry Heat
81
mode of action: burning contaminants into ashes
direct flaming
82
used for inoculating loops/needles
direct flaming
83
most common method of treating infectious waste
incineration
84
burning materials to ashes at temperatures 890°C-980°C
incineration
85
kills microorganisms by coagulation of proteins (denaturation)
MOIST HEAT
86
kills vegetative forms of bacterial pathogen
BOILING
87
mode of action: protein denaturation within 15 mins
BOILING
88
Effective for viruses and fungi, (less/more?) effective on endospores
BOILING, less
89
use of temperature up to 100°C at normal atmospheric pressure
FLOWING STEAM
90
mode of action of flowing steam
protein denaturation
91
Autoclaving - use of steam at
15 psi, temp of 121°C for 15 minutes
92
mode of action - irreversible denaturation of engines and structural proteins
Autoclaving

Clinical Bacteriology (5 decks)

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