Staining

Question 0

What is a differential stain?

Answer 0

-Gram stain positive or negative

Question 1

What is a simple stain?

Answer 1

One color

Question 2

What type of differential stain is used for ones that don’t stain well?

Answer 2

Acid-fast stain

Question 3

What type of differential stain is uses malachite green?

Answer 3

Endospore Stain

Question 4

Stain procedure:

Answer 4

Crystal violet- cells stained purple
Iodine- acts as mordant, cells remain purple
Ethanol/acetone- decolorized, gram positive- purple, negative-colorless
Safranin- positive remain purple, negative turns pink

Question 5

What does staining increase?

Answer 5

Contrast and resolution by coloring specimens w/ dye

Question 6

What do microbiological stains contain?

Answer 6

Chromophore (colored portion of dye)

Question 7

What are acidic dyes?

Answer 7

Acidic dyes stain alkaline structures

Question 8

What are basic dyes?

Answer 8

(common) Stains acidic structures

Question 9

What are special stains?

Answer 9

Negative (capsule) stain

Flagellar stain