Microscope

Question 0

What helps the intensity between objects or object and background?

Answer 0

Contrast

Question 1

What distinguish objects that are close together?

Answer 1

Resolution

Question 2

What helps determine resolution?

Answer 2

Contrast

Question 3

What increases contrast?

Answer 3

Staining and light in phase

Question 4

List other portions of microscope

Answer 4

Wavelength of radiation
Magnification
Light

Question 5

Radiation Scale (low to high)

Answer 5

Purple 400 nm Gamma rays —> Red 700 nm Radio waves/TV

Question 6

What kind of microscope has simple and compound?

Answer 6

Bright-field

Question 7

What type of Bright field has single lens?

similar to magnifying glass

Answer 7

Bright field Simple

Question 8

What Bright Field microscope has a series of lenses?

Light passes through specimen into objective lens
One or two ocular lenses

Answer 8

Bright field Compound

Question 9

What increases resolution in compound bright field?

Answer 9

Oil Immersion

most have a condenser lens (direct light)

Question 10

Magnification

Answer 10

Total mag= mag of objective lens X mag of ocular lens

Question 11

What kind of microscope is best for pale objects?

Answer 11

Dark-field

Question 12

Describe dark-field

Answer 12

• Light Rays scattered enter objective
• Specimen appears light against dark background
• Increases contrast/enables observations for details

Question 13

What kind of microscope can be divided into three different microscope?

Answer 13

Light Microscopy

Question 14

What kind of microscope:

• Examine living organisms that would be damaged if put on slide
• Light rays IN phase–brighter image
• Types: Phase contrast/differential interference contrast

Answer 14

Phase Microscope (light microscopy)

Question 15

What kind of microscope:

• Direct UV light
• Radiates energy back, visible wavelength
• UV light increases resolution/contrast
• Staining

Answer 15

Fluorescent Microscope (Light Microscopy)

Question 16

What kind of microscope:

• Fluorescent dyes
• UV lasers to illuminate fluorescent chemicals, single plane
• Resolution increase light passes pinhole
• Computer image

Answer 16

Confocal Microscope (Light Microscopy)

Question 17

What kind of microscope has a greater resolving power?

10,000 X to 100,000 X magnifies

Answer 17

Electron Microscopy

Question 18

What can you view with an electron microscopy?

Answer 18

Bacteria, viruses, internal cellular structures, molecules, atoms

Question 19

What types of electron microscopy are there?

Answer 19

Transmission electron

Scanning electron

Question 20

List the parts to a Microscope

Answer 20

• Ocular lens
• Body
• Arm
• Objective lens
• Stage
• Condenser
• Diaphragm
• Illuminator
• Coarse focusing knob
• Fine focusing knob
• Base

Question 21

Part of microscope: re-magnifies the image

Answer 21

Ocular lens

Question 22

Part of microscope: transmits image from objective lens to ocular

“Prism”

Answer 22

Body

Question 23

Part of microscope: Primary lens magnify specimen

Answer 23

Objective lens

Question 24

Part of microscope: Holds slide in position

Answer 24

Stage

Question 25

Part of microscope: Focuses light

Answer 25

Condenser

Question 26

Part of microscope: Controls amount of light entering condenser

Answer 26

Diaphragm

Question 27

Part of microscope: Light source

Answer 27

Illuminator

Question 28

Part of microscope: moves stage up and down

Answer 28

Coarse focusing knob