Staining

Question 1

Staining conn tissue and cytoplasm

Background stain

Counterstain

Answer 1

Eosin

Question 2

Eosin types

Answer 2

Eosin Y (yellowish) - common
Bluish (Eosin B, Erythrosin B)
Ethyl Eosin (Eosin S, Eosin OH soluble)

Question 3

Useful as progressive stain

Nuclear stain

Demonstrate cytoplasmic glycogen

Used when acid-OH diff might destroy/decolorize cytoplasmic comp

Used in Celestine Blue hemalum mtd nuclear staining

Answer 3

Mayer’s hematoxylin

Question 4

For diff or regressice staining

Use acid OH as diff agent

Answer 4

Iron hematoxylin soln

Question 5

Diff of muscle fiber and conn tissue

Standard hematoxylin

Uses ferric Ammonium

Answer 5

Weigert’s soln

Question 6

Uses ferric ammonium sulfate

Answer 6

Heidenhan’s soln

Question 7

Mordant: 1% aqueous phosphotungatic acid

Paraffin, celloidin, frozen sections

Answer 7

PTAH Phosphotungstic acid hematoxylin

Question 8

Red
Eosin Y B
Phioxine B

Answer 8

Cytoplasmic stain

Question 9

Yellow
Picric acid
Orange G
Rose bengal

Answer 9

Cytoplasmic stain

Question 10

Green
Light green sf
Lissamine green

Answer 10

Cytoplasmic stain

Question 11

Red
Neutral red
Safranin O
Hematoxylin
Carmine

Answer 11

Nuclear stain

Question 12

Toluidine blue
Nile blue
Celestine blue

Answer 12

Nuclear stain

Question 13

Gold chloride
Silver nitrate

As precipitates and not absorbed

Answer 13

Metallic stain

Question 14

Saffron
Hematoxylin
Orcein
Cochineal

Answer 14

Natural dye

Question 15

Basic dye

Thizine and triphenylmethane group

Answer 15

Metachromatic dye

Question 16

Methyl violet
Crystal violet
Cresyl blue retics
Bismarck brown
Safranin
Basic fushin
Toluidine blue
Thionine
Azure a b c
Methylene blue

Answer 16

Metachromatic stains

Question 17

Use of specific dyes

Diff particular subs

Metachromasia

For cartillage, conn tissue, eph mucis, mast cells, amyloid

Answer 17

Metachromatic staining

Question 18

For conn tissue

Answer 18

Acid fuschin-picric acid

van gieson stain

Question 19

Stains collagen, smooth muscle, mitochondria

Nuclear and cytoplasmic stain in mallory’s trichrome mtd

Answer 19

Acid fushin (masson stain)

Question 20

Most commonly used supravital stain

Answer 20

Neutral red

Question 21

For mitochondria

Answer 21

Janus green

Question 22

For dead cells

Answer 22

Trypan blue

Question 23

Toluidine blue
Thionine
Nile blue

Answer 23

Supravital (nucleus)

Question 24

Carmine
Lithium
India ink

Answer 24

Intravital

Question 25

Anilline or coal tar dyes

Answer 25

Synthetic dye

Question 26

Discrimination bw dead and living cells DNA Green fluorescence RNA Red

Answer 26

Acridine orange

Question 27

Demonstrates Ca salts and possible site of phospatase act

Answer 27

Acridine Red 3B

Question 28

Stains acid mucopolysaccharides | Excellent striking blue color

Answer 28

Alcian blue

Question 29

Orange red lake w/Ca at pH4.2 Used on Dupont ACA analyzer measure serum Ca

Answer 29

Alizarin Red S

Question 30

Counterstaining eph sections

Answer 30

Aniline blue

Question 31

Plasma stain For AFOrganism, mitochondria, diff of smooth muscle w/picric acid Main constituent of Feulgen’s acid and Schiff’s rgt - aldehydes

Answer 31

Basic fuschin

Question 32

For conn tissue, mucin, elastic tissue

Answer 32

Van gieson’s soln

Question 33

For staining hgb

Answer 33

Benzidine

Question 34

Contrast stain for gram’s tech In acid fast In pap mtd For diptheria organism

Answer 34

Bismarck brown

Question 35

For fresh materials in smear prep Det presence of metal ions eg Al

Answer 35

Carmine

Question 36

Mordanted dye Acts as basic dye and stains acid subs

Answer 36

Carmalum (mayer’s soln)

Question 37

Alt to iron hematox

Answer 37

Celestine blue

Question 38

Best known as indicator Stain for axis cylinders in embryos Identify chon depo (amyloid)

Answer 38

Congo red

Question 39

In histo for nervous tissue

Answer 39

Cresyl violet

Question 40

Stains amyloid in frozen sections and plts

Answer 40

Crystal violet

Question 41

For metallic impregnation

Answer 41

Gold sublimate

Question 42

Oldest stain

Answer 42

Iodine

Question 43

Identify and diff bacteria

Answer 43

Gram’s iodine

Question 44

Decolorizer Counterstain Weakly basic dye Contrast stain for ascaris eggs and rbc Bacterial spore stain

Answer 44

Malachite green

Question 45

Formed by boiling nile blue w/sulfuric acid For living cells Accumulates in lipid globules inside cells - red

Answer 45

Nile red (nile blue oxazone)

Question 46

More soluble in fat than H2O or OH For neutral lipids

Answer 46

Oil red O

Question 47

For elastic fibers In derma studies Finest and most delicate skin fiber demonstration

Answer 47

Orcein

Question 48

Selective stain for unsaturated lipids For lipoCHONs Myelin=black

Answer 48

``` Osmic acid Osmium tetroxide (OsO4) ```

Question 49

``` All around stain Glycogen Mucin MucoCHON GlyCHON Basement memb Capsules Blood vessels Fungi Intra carbs glycogen in hepatocytes ```

Answer 49

Periodic acid schiff

Question 50

Common neg stain for viruses, nerves, polysaccharides For striated muscle fibers Mitochondria = blue

Answer 50

Phosphotungstic acid

Question 51

``` Contrast stain to acid fuschin Cytoplasmic stain Counterstain to crystal violet Tissue fixative Decalcifying agent ```

Answer 51

Picrid acid

Question 52

Used w/ osmic acid to fix and stain blood and glandular tissues

Answer 52

Rhodamine B

Question 53

Most senstive | Greater affinity to phospholipids than others lysochromes

Answer 53

Sudan black

Question 54

Not real dyes | Do not have auxochrome groups

Answer 54

Lysochromes (Oil soluble dyes)

Question 55

No 2ndary amino grp Does not color phospholipids For TAG

Answer 55

Sudan IV

Question 56

First introduced Fat soluble Good fat stain for CNS

Answer 56

Sudan III

Question 57

Binds collagen in EM = pink

Answer 57

Van gieson stain

Question 58

For neuroglia in frozen section

Answer 58

Victoria blue

Question 59

Silver reduction mtd For phosphates and carbonates Usually present along Ca

Answer 59

Von kossa stain

Question 60

Causes blood cells to exhibit 4 major staining properties

Answer 60

Wright stain