Staining Part 1

Question 1

This staining method will stain iron pigments bright blue, nuclei red, and cytoplasm pink to rose.

Answer 1

Gomori’s Prussian blue Stain for Iron

Question 2

This is used for differential staining of pancreatic islets of Langerhans.

Answer 2

Gomori’s Aldehyde Fuchsin Stain

Question 3

A tissue deposit that can be removed from the tissues by washing
them out from sections with the use of acid alcohol.

Answer 3

Chrome

Question 4

This staining method with the reaction that is not melanin-specific and may stain other
elements, such as argentaffin, chromaffin cells, and some types of lipofuscin.

Answer 4

MASSON-FONTANA

Question 5

The best sections to use in staining glycogen using Best Carmine Method.

Answer 5

Celloidin

Question 6

The following fixatives are recommended for glycogen staining, EXCEPT:
alcohol formol, picric acid, acetic acid, gendre’s solution

Answer 6

picric acid

Question 7

This is used for differential staining of pancreatic islets of Langerhans.

Answer 7

Gomori’s Aldehyde Fuchsin Stain

Question 8

It is the most popular method for general demonstration of acid
mucopolysaccharides, using 3% acetic acid at pH 2.5.

Answer 8

Alcian Blue Technique, pH 2.5

Question 9

It is ideal for demonstrating striated muscle fibers and mitochondria, often without a
counterstain

Answer 9

MALLORYS PTAH

Question 10

It is the counterstain used in Best Carmine Method

Answer 10

Ehrlich’s hematoxylin

Question 11

This staining method consists essentially of immersing fresh pieces of nervous tissue first
in a solution of potassium dichromate and then in silver nitrate.

Answer 11

Golgi’s Silver Staining technique

Question 12

Using this staining method, neurofibrils, axons and dendrites stain black.

Answer 12

Cajal’s Gold Sublimate Method for Astrocytes

Question 13

This staining method can diagnose malignancies such as rhabdomyosarcomas and
hemangiosarcomas thru reticulin patterns and can also aid in distinguishing between
epithelial and non-epithelial cancers.

Answer 13

Gomori silver staining

Question 14

The color of muscle tissues when stained with Van Gieson

Answer 14

YELLOW

Question 15

It is the main storage form of glucose, manufactured and stored chiefly in the liver, but is also found normally in less quantity in muscles, parathyroid and cartilage.

Answer 15

glycogen

Question 16

This is considered as the method of choice for glycogen staining.

Answer 16

PAS w DIASTASE

Question 17

This staining method relies upon binding with the tetrazolium salt and converts it into an insoluble formazan compound. It also provides an excellent stain of the intermyofibrillary matrix–the space between the myofibrils.

Answer 17

NADH STAIN

Question 18

The simplest method of differential staining of collagen using a mixture of Picric Acid and
Acid Fuchsin.

Answer 18

Van Gieson

Question 19

It is soluble in strong alkali, and does not react with iron or fat stain.

Answer 19

Melanin

Question 20

This staining method is used to identify contraction bands as seen in contraction band necrosis

Answer 20

Mallorys PTAH

Question 21

They stain red with PAS but do not stain with Alcian blue, colloidal iron, mucicarmine, or metachromatic dyes

Answer 21

NEUTRAL MUCOPLY

Question 22

The ideal location of muscle tissue in obtaining samples for a needle biopsy.

Answer 22

Thigh

Question 23

It is a rapid method for staining fibrin, elastic fibers and amyloid. It stains the fibrin dark
blue and leastic fibers bright red.

Answer 23

Krajian

Question 24

This is the oldest stain, and is already considered obsolete because it is not specific for glycogen, also coloring Amyloid and other protein substances

Answer 24

Langhan’s

Question 25

This stain is used for demonstrating neuritic plaques and neurofibrillary tangles to diagnose Alzheimer’s disease.

Answer 25

Bodian’s

Question 26

Before staining with PTAH for astrocytes demonstration, mercuric deposits should be removed by

Answer 26

IODINE

Question 27

It is the only large group of carbohydrate compounds that are not strongly PAS positive

Answer 27

ACID MUCOPOLY

Question 28

This technique when demonstrating calcium involves replacement of the anionic part of calcium salts with silver.

Answer 28

Von Kossa

Question 29

The staining method that preserves the morphology of muscle tissues well and allows for long-term storage at room temperature but is cumbersome and requires the handling of toxic chemicals.

Answer 29

h n e

Question 30

The presence of this color indicates a PAS positive reaction.

Answer 30

magenta

Question 31

It is the primary stain used for most bone biopsies.

Answer 31

hne

Question 32

A staining technique that can demonstrate melanin.

Answer 32

masson fontana for melanin

Question 33

A staining method that is useful for detecting ragged fibers in mitochondrial myopathy.

Answer 33

gomori

Question 34

The reaction of this method depends upon progressive oxidation of Bilirubin to blue-green Biliverdin by addition of Nitric Acid, changing the yellowish-brown pigment to blue and purple; then to green color within seconds.

Answer 34

gmelin

Question 35

Hemosiderin and ferric salts stain deep blue in this staining method.

Answer 35

perl's prussian

Question 36

Using this type of stain in liver biopsy samples, we can assess the degree of fibrosis in the liver tissue such as in chronic liver disease, and cirrhosis

Answer 36

masson

Question 37

Using Van Gieson stain, it will impart what color on the muscles?

Answer 37

yellow