Staining Part 2 Flashcards
(32 cards)
This staining method stains the free fatty acids dark green.
Copper rubeanic acid
A smear preparation that is useful for the assessment of cellularity, megakaryocyte
number, focal diseases and fibrotic marrows.
squash smear
If tuberculosis or fungal infection is suspected, the fixed sample should be placed in
formalin for how long?
48h
It stain the bile pigments green and collagen, muscle and bone pale pink.
giemsa
The following are the different dyes used in fluorescent staining for DNA and RNA,
EXCEPT:
rhodamine, fluorochromes, acriflavine, acridine red
acridine red
This method is used for nuclear sex determination and for general morphological studies.
feulgen
Which of the following statements best describes Sudan Black B:
most sensitive
It is useful for the assessment of overall marrow architecture and cellularity and provide
greater sensitivity for the assessment of focal lesions and patchy infiltrates.
trephine
The following are techniques in staining nucleic acids, EXCEPT:
feulgen, alcian blue, gallocyanin, methyl green
alcian blue
It is used to remove nucleic acid that may mask the
basic group of protamines and histones.
trichloroacetic acid
It can be demonstrated by selective staining with small or large-molecule dyes using the
Trichrome method
fibrous proteins
It can serve as a sensitive vital stain for the detection of cytoplasmic lipid droplets
nile red
It is the gold standard for histologic interpretation of lymph node specimen.
h&e
In Feulgen’s method, it is used to remove the excess Schiff’s reagent from the section,
while preventing its oxidation back to basic fuchsin
bisulfite solution
A staining method to identify plasma cells, mast cells, lymphoid cells, eosinophils, and for
distinguishing between myeloblasts and proerythroblasts.
giemsa
This method can give the DNA a green fluorescence while RNA is stained brick to
orange-red.
fluorescent staining
It is the most commonly used fluorochrome to demonstrate DNA and RNA in fresh or
fixed tissues, combining with nucleic acids in cells by salt linkages and cohesion.
acridine orange
the following are advantages of microwave ovens, except:
greater profitability, increased efficiency, temperatures maintained at 75-80 C, shorter processing
temp
Bone marrow must be fixed with Zenker’s fluid and acetic acid for how long before
processing?
14-24
A staining method to demonstrate both DNA and RNA in nucleic acids.
gallocyanin
A temperature that can speed up fluid penetration and tissue processing in general.
37-45
A dye that emits light or visible radiation energy when excited by the light of shorter
wavelength, either visible or ultraviolet.
fluorescent
The preferred staining method for bone marrow aspirate smears
wright
The preferred staining method for bone marrow aspirate smears
myeloperoxidase
