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Flashcards in Stains Deck (31)
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1

Name 2 special stains for dermatophytes or other fungi.

1. Gomori methenamine silver (GMS)
2. periodic acid-Schiff (PAS)

2

What color does GMS stain dermatophytes or fungi?

black

3

What color does PAS stain fungi?

magenta-purple

4

Name a stain for fats/cholesterol clefts.

Oil red O

5

What color does Oil red O stain fats/cholesterol clefts?

red

6

Name 2 special stains for elastic fibers.

1. Verhoeff
2. Van Gieson

7

What color does Verhoeff stain elastic fibers?

red

8

What color does Van Gieson stain elastic fibers?

black- binds to elastin within elastic fibers

9

Name a stain for calcinosis cutis/calcium.

Von Kossa

10

What color does Von Kossa stain calcium?

metallic silver?/black

11

Name 3 special stain for melanin.

1. Fontana-Masson
2. Grimelius
3. GMS

12

What color do Fontana-Masson, grimelius and GMS stain melanin?

black

13

Name a special stain for amyloid.

Alkaline Congo red

14

What color does Alkaline Congo red stain amyloid?

red

15

Name a special stain for collagen.

Masson's trichrome

16

What color does Masson's trichrome stain abnormal collagen?

red (blue=normal)

17

Name 2 special stains for Mast cells.

1. Toluidine blue
2. Acid orcein-giemsa

18

What color do Toluidine blue and giemsa stains stain mast cells?

purple red metochromatic

19

Name a special stain for the capsule of Cryptococcus neoformans in tissue samples.

Mayar's mucicarmine

20

What color does Mayar's mucicarmine stain the Cryptococcus capsule?

red

21

Name a special stain for reticular fibers.

Gomori's methenamine silver

22

What color does GMS stain reticular fibers?

black

23

Name 2 special stains for glycosaminoglycans.

1. Alcian blue
2. Toluidine blue

24

What color does Alcian blue and toluidine blue stain glycoaminoglycans?

1. Alcian blue: blue-green
2. Toluidine blue: purple

25

Name a special stain for eosinophils.

Giemsa

26

What color does Giemsa stain eosinophils?

nucleus= blue/purple
cytoplasmic granules: red

(aka purple red metachromatic)

27

Name a special stain for mycobacteria.

Ziehl-Neelsen

28

What color does Ziehl-Neelsen stain mycobacteria?

red

29

Describe the process of Ziehl-Neelsen staining.

1. carbol fuschin is applied and stains all cells red
2. acid alcohol is applied and de-stains all non-acid-fast cells as they lack thick waxy lipid wall (which holds in carbol fuschin)
3. blue counterstain is applied staining all non-acid-fast blue

30

Name a special stain for the BMZ.

periodic acid-Schiff