Flashcards in Stains Deck (31)
Name 2 special stains for dermatophytes or other fungi.
1. Gomori methenamine silver (GMS)
2. periodic acid-Schiff (PAS)
What color does GMS stain dermatophytes or fungi?
What color does PAS stain fungi?
Name a stain for fats/cholesterol clefts.
Oil red O
What color does Oil red O stain fats/cholesterol clefts?
Name 2 special stains for elastic fibers.
2. Van Gieson
What color does Verhoeff stain elastic fibers?
What color does Van Gieson stain elastic fibers?
black- binds to elastin within elastic fibers
Name a stain for calcinosis cutis/calcium.
What color does Von Kossa stain calcium?
Name 3 special stain for melanin.
What color do Fontana-Masson, grimelius and GMS stain melanin?
Name a special stain for amyloid.
Alkaline Congo red
What color does Alkaline Congo red stain amyloid?
Name a special stain for collagen.
What color does Masson's trichrome stain abnormal collagen?
Name 2 special stains for Mast cells.
1. Toluidine blue
2. Acid orcein-giemsa
What color do Toluidine blue and giemsa stains stain mast cells?
purple red metochromatic
Name a special stain for the capsule of Cryptococcus neoformans in tissue samples.
What color does Mayar's mucicarmine stain the Cryptococcus capsule?
Name a special stain for reticular fibers.
Gomori's methenamine silver
What color does GMS stain reticular fibers?
Name 2 special stains for glycosaminoglycans.
1. Alcian blue
2. Toluidine blue
What color does Alcian blue and toluidine blue stain glycoaminoglycans?
1. Alcian blue: blue-green
2. Toluidine blue: purple
Name a special stain for eosinophils.
What color does Giemsa stain eosinophils?
cytoplasmic granules: red
(aka purple red metachromatic)
Name a special stain for mycobacteria.
What color does Ziehl-Neelsen stain mycobacteria?
Describe the process of Ziehl-Neelsen staining.
1. carbol fuschin is applied and stains all cells red
2. acid alcohol is applied and de-stains all non-acid-fast cells as they lack thick waxy lipid wall (which holds in carbol fuschin)
3. blue counterstain is applied staining all non-acid-fast blue