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Flashcards in Stains- Lab 4 Deck (28)
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1

What shape is a coccus?

Circle

2

What does the gram stain, stain?

The cell NOT PG

3

What technique does the gram stain use?

Gram stain

4

Name the steps for gram stains

1. Differential stain
2. Primary stain: crystal violet
3. Counter stain: safranin red

5

What color is a gram positive?

Purple

6

What color is a gram negative?

Red

7

Name the gram positive organism tested in lab

Bacillus subtilis (rods)

8

Name the gram negative organism tested in lab

Escherichia coli (E. coli) (rods)

9

What does the endospores stain, stain?

Endospores (vs. vegetative cells)

10

Name the steps of the crystal violet method (endospores)

1. Negative stain for endospores
- crystal violet- purple vegetative cells
- endospores - clear

11

Name the steps for the Schaeffer-Fulton method (endospores)

1. Special stain
- endospores- green
- Vegetative cells - red

12

What was the organism tested in the endospore stain?

Bacillus subtilius

13

What is stained in the acid-fast stain?

Mycolic acid

14

What technique does the AF stain use?

Ziegler-Neelsen technique

15

What methods does the endospore stain use to stain ?

Crystal violet method & Schaeffer- Fulton method

16

Name the steps in the Ziehl-Neelsen technique (AF)

1. Differential stain
2. Primary stain: Carbol fuchsin
3. Counterstain: methylene blue

17

What color is a AF?

Pink

18

What color is a non-AF?

Blue

19

Name the organisms tested in lab for AF stain

1. Mycobacterium smegmatis (AF rods)
2. Staphylococcus epidermis (non-AF cocci)

20

What does the capsule stain, stain?

Capsule

21

What method does the capsule stain use?

Capsule stain

22

Name the steps of the capsule stain

1. Special stain
2. Negative stain for capsule
- India ink: black background
- capsules: clear
- cells: red

23

What does the flagella stain, stain?

Flagella

24

What technique does the flagella stain use?

Leifsons technique or flagella stain

25

Name the steps of the flagella stain

1. Special stain
- Mordant adheres to flagella
- rolling drop slide

26

What was the organism used in the flagella stain lab

Proteus vulgaris

27

What is the only stain that had mixed culture? (Rods& coccus)

Acid fast stain

28

What shape is a rod?

Rod