staphylococcus, micrococcus & related organisms

Question 1

test to differentiate staphylococci & micrococci from streptococci

Answer 1

catalase test

Question 2

catalase test reagent

Answer 2

3% hydrogen peroxide

Question 3

use of colonies from BAP for catalase test will result to

Answer 3

false positive result

Question 4

(+) result for catalase

Answer 4

bubbles

Question 5

grape like clusters

Answer 5

staphylococci

Question 6

tetrads/cuboidal packets

Answer 6

micrococci

Question 7

definitive test for S. aureus

Answer 7

coagulase test

Question 8

reagent/media for coagulase test

Answer 8

rabbit’s plasma collected using EDTA

Question 9

use of citrated plasma for coagulase test will cause

Answer 9

false positive

Question 10

detects the ability of the organism to produce acid

Answer 10

fermentation

Question 11

MSA inhibitor

Answer 11

7.5% salt

Question 12

MSA indicator

Answer 12

phenol red

Question 13

DNAse is AKA

Answer 13

thermonuclease test

Question 14

0.1% HCl precipitation method positive result

Answer 14

clearing of agar around the colonies

Question 15

dye method for coagulase uses

Answer 15

toluidine blue OR methyl green

Question 16

clear zone around the colonies
A. methyl green
B. toluidine blue

Answer 16

A

Question 17

pink zone around the colonies
A. methyl green
B. toluidine blue

Answer 17

B

Question 18

S. aureus VP__ & PYR__

Answer 18

VP (+)
PYR (-)

Question 19

coagulase negative staphylococci

Answer 19

S. epidermidis & S. saprophyticus

Question 20

gram positive, bacilli, aerobic

Answer 20

listeria, bacillus, corynebacterium

Question 21

gram positive, bacilli, anaerobic

Answer 21

clostridium

Question 22

gram positive, cocci, catalase positive, coagulase positive

Answer 22

S. aureus

Question 23

gram positive, cocci, catalase positive, coagulase neg, novobiocin resistant

Answer 23

S. saprophyticus

Question 24

gram positive, cocci, catalase positive, coagulase neg, novobiocin susceptible

Answer 24

S. epidermidis

Question 25

gram positive, cocci, catalase negative, alpha hemolytic, optochin resistant

Answer 25

viridans

Question 26

gram positive, cocci, catalase negative, alpha hemolytic, optochin susceptible

Answer 26

S. pneumoniae

Question 27

gram positive, cocci, catalase negative, beta hemolytic, bacitracin resistant

Answer 27

S. agalactiae (group B)

Question 28

gram positive, cocci, catalase negative, beta hemolytic, bacitracin susceptible

Answer 28

S pyogenes (group A)

Question 29

gram positive, cocci, catalase negative, gamma hemolytic, 6.5% NaCl growth

Answer 29

enterococci (group D)

Question 30

gram positive, cocci, catalase negative, gamma hemolytic, NO GROWTH on 6.5% NaCl

Answer 30

non enterococcus
(S. bovis)

Question 31

aerobic, weakly acid fast

Answer 31

nocardia

Question 32

anaerobic, not acid fast

Answer 32

actinomyces

Question 33

DNAse test positive organisms

Answer 33

serratia, moraxella, S.aureus (SMS)

Question 34

prosthetic heart valve infection

Answer 34

S. epidermidis