Steps of Transcription - termination

Question 1

Step 1 - termination

Answer 1

CstF and CPSF bind CTD of RNA polymerase and are transferred to the processing sequence own the mRNA transcript as it emerges from the polymerase.

Question 2

Step 2 - termination

Answer 2

mRNA is cleaved 10-30 bp downstream of processing sequence.

Question 3

Step 3 - termination

Answer 3

Poly (A) tail is added by Poly A Polymerase on the 3’ end

Question 4

Step 4 - termination

Answer 4

Poly A binding proteins bins Poly A tail.

Question 5

Step 5 - termination

Answer 5

RNA polymerase continues transcribing until is dissociates from DNA.

Question 6

Step 6 - termination

Answer 6

The second RNA fragment produced after cleavage is degraded.

Question 7

What is the purpose of polyadenylation?

Answer 7

Polyadenylation increases the half life and is important for export of the mRNA.