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Flashcards in Sterilisation Deck (12)
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1

Autoclave use

Adequate spacing
Inlet & exhaust valves not blocked
Distilled water
Instruments free of grease & organic matter

2

Principle of heat sterilisation

Steam applied under pressure for specific time
134c

3

Cold sterilisation, how does it work & principle

Ethylene oxide gas
Inactivated DNA of pathogenic organisms
Adequate temp/time/pressure
As temp increases ability of gas to penetrate increases - shorter cycle

4

Chemical indicator strips

Colour change
Correct temp/pressure/time

5

Browne’s tubes

Colour change (red-green)
Correct temp maintained for specific period of time

6

Bowie-dick tape

Colour change when certain temp reached
Not indicator of sterility

7

Shore tests

Strips of paper impregnated with dried bacterial spores
Following cycle, cultured & incubated for 3 days

8

Principles of packing

Must not damage equipment
Must be resistant to handling damage
Must be permeable to steam/gas
Microorganisms must not be able to penetrate from outer surface

9

Sterilisation area/sterile storage

Close to theatre; away from sterile storage
Sterile supplies in closed cupboard; adjective to theatre; away from instrument cleaning area

10

Items which can be autoclaved

Instruments
Drapes/gowns/swabs
Glassware
Most non-disposable plastic/rubber items

11

Items usually cold sterilised

Fibre optics
Optical equipment
Battery operated drills
Disposable plastics
Anaesthetic tubing

12

Sterilisation steps

PPE
Soak & scrub; purpose specific brush - jaws open
Instrument cleaner prepared as per MG
Rinse running water 3mins
Ultrasonic cleaner (MG) rinse 3 mins
Dry; check for damage; lubricate
Protective cover
Package correct way round; ratchets open
Insert indicator; remove air; seal
Double wrap; label w/permanent marker