Sterilization and Disinfection Flashcards
(35 cards)
Removal or Destruction of all forms of life
a. Sterilization
b. Disinfection
a. Sterilization
disinfection - removal, INHIBITION OR KILLING OF MICROORGANISMS, although it DOES NOT REMOVE ALL BACTERIAL SPORES
is the Most Commonly Used Method for removal of microorganisms
a. heat
b. Filtration
c. radiation
a. heat
FASTEST AND SIMPLEST method of sterilization, killed within 15 mins
a. boiling
b. autoclaving
c. inspissation
b. autoclaving
it destroy vegetative bacteria (non-spolurating)
100*c for 10-15mins
a. boiling
b. autoclaving
c. inspissation
a. boiling
used to sterilize BIOHAZARDOUS TRASH AND HEAT STABLE OBJECTS
A. autoclaving
b. boiling
c. fractional
a. autoclaving
used is steam under pressure
a. autoclaving
b. fractional
c. pasteurization
a. autoclaving
the instrument used in FRACTIONAL OR INTERMITTENT STERILLIZATION
a. Arnold’s sterilizer
b. Anton sterilizer
c. aton sterilizer
a. Arnold sterilizer
performed at 100*C for 30 mins for 3 consecutive days
a. fractionation/ intermittent sterilization
b. inspissation
a, fractionation or intermittent sterilization
performed at 70C to 80C for 2 hours for 3 consecutive days
a. fractionation/ intermittent sterilization
b. inspissation
b. inspissation
used to sterilize PROTEIN RICE MEDIUM (LJ MEDIUM)
a. boiling
b. inspissation
b. autoclaving
b. inspissation
destroys vegetative cells and spore for 3 consecutive days of sterilization
fractionation or intermittent sterilization
it is used to sterilize MILK, DAIRY PRODUCTS AND ALCOHOLIC BEVERAGES
pasteurization
quick heating then immediate cooling ( 72*C for 15 seconds)
a. low temperature holding/ batch method
b. high temperature short time (HTST)/ flash pasteurization
c. ultra high temperature (UHT)
b. high temperature short time (HTST)/ flash pasteurization
cooled very quickly in a vacuum chamber (140*C for 3 secs)
a. low temperature holding/ batch method
b. high temperature short time (HTST)/ flash pasteurization
c. ultra high temperature (UHT)
c. ultra high temperature (UHT)
sterilization method that DOES NOT REQUIRE WATER
a. moist heat procedure
b. dry heat procedure
b. dry heat procedure
utilized for sterilization of glassware, oil products and powder
a. moist heat
b. dry heat
c. filtaration
b. dry heat
biological indicator of dry heat procedure?
a. Bacillus atrophaeus
b. Bacillus stearothermophilus
c. Bacillus subtilis var. niger
a&c Bacillus atrophaeus ( Bacillus subtilis var. niger)
bilogical inidcator of autoclaving
a. Bacillus atrophaeus
b. Bacillus stearothermophilus
b. Bacillus stearothermophilus / Geobacillus stearothermophilus
most common method of treating infectious waste and infected laboratory animals
a. oven heating
b. flaming
c. incineration
c. incineration
what is the temperature used in HAZARDOUS MATERIAL?
a. 870C to 980C
b. 300 to 400C
c. 160 to 170C
a. 870C to 980C
used to control the spread of communicable disease
a. flaming
b. oven heating
c. incineration
d. cremation
cremation
it is the method of choice for STERILIZATION OF ANTIBIOTIC SOLUTIONS, TOXIC CHEMICALS, RADIOISOTOPES, VACCINES AND CARBOHYDRATES
a. sterilization
b. disinfectants
c. filtration
c. filtration
it is a type of filter that is made up FIBROUS OR GRANULAR MATERIALS
a. depth filter
b. hepa filter
c. membrane filter
a. depth filter
This type of filter is used to sterilize PHARMACEUTICALS, OPTHALMIC SOLNS, CULTURE MEDIA, ANTIBIOTICS AND OIL PRODUCTS
a. depth filter
b. hepa filter
c. membrane filter (circular filter)
c. membrane filter (circular filter)