Still Forgetting Flashcards
(25 cards)
Why each amino acid has a different Rf value
Each amino acid has different affinity/attraction/solubility to stationary and mobile phase
Structural features of enzyme enabling it to catalyse hydrolysis of isomer G but not isomer F
Enzyme has active site
Isomer had correct stereochemistry
Suitable reagent for hydrolysis of protein
Conc hcl
What to include when drawing dna
Bonds on deoxyribose
On LHS of DNA deoxyribose molecule
Phosphate ion sugar on right
Why interaction between A+T differ from previous
Only has 2H bonds in AT base pair
Why urea is effective in deprecating complementary strands in DNA
amino/NH2 group in urea is able to substitute for H in double helix
Interactions in 2° protein
Both Oxygen and Nitrogen are very electronegative meaning C=O and N-H are polar. Leads to firbation of H bonds between P and H . The Lone pair of e- on O atom is very strongly attracted to H(+ve)
What is affected at low Ph
N-H group has all Hs and +ve ion and acid is unaffected therefore is COOH
Minimise side effect if cis platin
Use in smaller quantities/target application to tumor
Why is chromatography done in fume cupboard
Solvent is toxic/hazardous
C=C bond
Double covalent bond consists of one sigma and one pi bond. Pi bond is exposed and gas high electron density. Therefore it is vulnerable to attack by species which like electrons known as electrophiles
E/Z isomers occur due to restricted rotation
Reducing nitrobenzene to aromatic amines (phenyl amine )
Sn or Fe and HCl
Delocalisation of CA
Stabilised by delocalisation which makes dissociation more likely. Delocalised ion has equal c-o lengths whereas usually C=O bond is shorter. Pi charge clouds is delocalised and spread out making ion more stable and likely to form
Why is ethanol added in small amounts
To avoid aspirin from dissolving and to remove any soluble impurities
Precaution with ethanol
Heat below its bp to avoid ethanols drum boiling away
Difference between crude and pure aspirin mp
Crude has lower mp value
Hazard of conc HPO3
Corrosive
Suitable apparatus for measuring out ethanoiic acid
Graduated pippete or burette
Why polyester biodegradable but not polyalkenea
Polyesters are polars d C=P bond whereas polyalkenes are non polar and C-C bonds
Filling birette
Below eye level
Dissolving solid
Gloves
