Structure

Question 1

SLAP1 Cbl binding sequence

Answer 1

hu - LRESASYLSLTSEDNTSF

ms- LRESASYLSLTGDDSSSF

Question 2

SLAP2

Answer 2

hu - LRESLSFYSLNDEAVSLD

ms- LRESLSSUSLAEDP__LD

Question 3

Comparison between SLAP1/2 Cbl binding

Answer 3

hu1 - LRES_ASYLSLTSEDNTSF
hu2- LRESLSFY_SLNDEAVSLD

ms1 - LRES_ASYLSLTGDDSSSF
ms2- LRESLSSY_SLAEDP__LD

Question 4

Chromosomal Location msSLAP1

Answer 4

Chromosome 15

Question 5

Chromosomal Location huSLAP1

Answer 5

Chromosome 8q22.3 embedded in the thyroglobulin gene locus

Question 6

Chromosomal Location msSLAP2

Answer 6

Chromosome 2

Question 7

Chromosomal location of huSLAP2

Answer 7

Chromosome 20q11.23

Question 8

SLAP2 size

Answer 8

256 AA, 25-28 kDA depending on splice variants

Question 9

SLAP2 isoforms

Answer 9

splice variants

Question 10

Identification of SLAP1/cbl interaction

Answer 10

Yeast-2-hybrid screen
Bait: residues 25-351 of c-Cbl
Prey: cDNA Library from PHA stimulated human leukocytes
Mapping shows that if you knock out the charged part of the SLAP1 c-terminal region

Question 11

Interaction between cbl and zap70

Answer 11

nterminal PTB domain of cbl and pY292 of Zap70

Question 12

SLAP1 size

Answer 12

276AA 34 kDa

Question 13

SLAP1 cTerminal region

Answer 13

aa210-241 = leucine rich hydrophobic region
aa240-276 = Highly charged (arginine C-CH2C00-) (Glutamic acid - C-CH2-CH2-COO) (Lys C-(CH2)4-NH3+) (Arginine C-(CH2)3-NH-CNH2NH2+)

Question 14

LOF mutation in SLAP1 SH2

Answer 14

R111K

Question 15

SLAP Preferential binding sequence

Answer 15

pY+3: Aliphatic residues (V,I,P)
pY+1: Small (A,S,T)
Acidic Residues (E,D,Q,H)

Question 16

ITAM Motif

Answer 16

18aa
YxxL/I-X(6-8)-YxxL/I
pY is position zero
The specificity of an SH2 domain for a given ITAM motif is determined by the flanking residues, usually at the -2 to +3