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1

What is muscle tissue specialized for? What are the 3 histologically distinct types of muscle?

1. specialized for contractility and excitability in order to perform movements. muscle cells are elongate and have membrane organelles specialized for contraction. 

2. skeletal: attach to bone and responsible for movement of body parts

cardiac

smooth: blood vessels, GI tract

2

Muscle contraction is also a source of ____.

heat

3

Muscle tissue can be striated or non-striated and voluntary or involuntary. What types of muscle fall into what categories?

skeletal: striated voluntary

cardiac: striated involuntary

smooth: non-striated involuntary

 

note: striated=light and dark bands on microscopic level

voluntary: under cerebral cortical control

involuntary: under autonomic nervous system control

4

What are the levels of structural organization of skeletal muscle? What is the functional unit of muscle?

1. muscle (for example deltoid) is composed of numerous muscle fasicles that are composed of bundles of muscle fibers/muscle cells. muscle fibers/muscle cells/myofibrils are composed of myofibrils (organelle with repeating sarcomere units) that is composed of myofilaments (actin and myosin myofilaments

2. sarcomere

5

What are characteristics of skeletal muscle cells? (include discussion of nuclei, organelles, cytoplasm) How are they formed?

1. each muscle cell/muscle fiber/myofiber is a multinucleated cell with numerous peripherally located nuclei just beneath the plasma membrane. the plasma membrane in muscle is called the sarcolemma. cytoplasm, called sarcoplasm in muscle, is dominated by contractile filaments but is also rich in mitochondria. the sarcoplasmic reticulum is a specialized form or smooth ER and surrounds myofilaments (actin and myosin). functions in Ca2+ storage

2. muscle cells are formed from the fusion of precursor cells called myoblasts which results in the long multinucleated fibers

 

6

What are the 3 connective tissue layers and what portions of muscle do they surround? What is their purpose?

epimysium: dense irregular connective tissue that surrounds all of the fasicles of a muscle (the entire muscle)

perimysium: thinner layer of dense irregular connective tissue that surrounds individual fasicles

endomysium: fine layer of reticular fibers that surround individual muscle fibers (cells)

connective tissue layers maintain the 3D structure of muscles and also serve as a route for nerve and circulatory supply to muscle tissue

7

8

How many skeletal muscle fiber types are there? How are they characterized?

3 diff skeletal muscle fiber types based on differences in contractile speed, metabolic profile, and fiber morphology. most muscles have all 3 types but proportion depends on function of a particular muscle

9

Myofibrils are composed of myofilaments. What are myofilaments?

individual polymers of myosin (thick filaments) and actin with its associated proteins (thin filaments)

10

What are the 3 major proteins of thin filaments?

1. actin: a globular protein that polymerizes in a double helical structure forming the thin filament

2. tropomyosin: protein that also forms a double helical structure that lies in the groove of the actin helix. acts with the troponin complex to regulate myosin-actin binding

3. troponin complex: composed of 3 globular subunits

  • TnI: actin binding protein that prevents binding of actin to myosin
  • TnT: tropomyosin binding protein that holde troponin complex to actin
  • TnC: calcium binding protein, essential step in initiation of muscle contraction

11

What are the 3 major proteins of thick filaments?

1. myosin heavy chain: large protein with 2 portions-an alpha helical "rod" portion and a globular "head" protion containing ATP and actin binding sites

2. myosin light chain 1

3. myosin light chain 2

light chain phosphorylation helps regulate calcium stimulation of contraction. is more important in smooth muscle

12

Myofilaments are linked into a highly structured organization called the ____, a unique structural feature of striated muscle. ____ (same as previous blank) line up end to end along the length of the muscle fiber in small parallel bundles called ____. The highly organaized arrangement of thick and thin filaments in a specific banding pattern forms the basis for muscle contraction

1. sarcomere

2. sarcomeres

3. myofibrils

13

True or false: There are additional accessory proteins that regulate spacing, attachment, and alignment of myofibrils. 

True.

14

Give definitions of each and be sure to discuss which change sizes during contraction and whether or not they contain overlap of filaments:

A (anisotropic) band

I (isotropic) band

Z-line or disc

M-line

H-band/zone

A-band: darker stained band seen in histological slides (dArK=A band) that is equivalent to the length of the myosin (thick) filament. It contains both the thick filament and the portion of the thin filament that overlaps. The extent of overlap depends on the degree of muscle contraction. Stays the same length regardless of muscle contraction!!!

I-band: lighter stained band (light=I-band) seen in histological slides and is equivalent to the portion of thin filaments in adjacent sarcomeres that are not overlapping with thick filaments. Shorten during contraction and elongate during muscle stretch!!!

Z-line/disc: serves as an anchor for thin filaments. defines boundaries of sarcomere (sarcomere is from one Z-line to the next)

M-line: located at center of sarcomere. where myosin filaments are anchored by accessory proteins

H-zone: distance between end of actin molecules from opposite ends of sarcomere (no overlap of actin & myosin). Shorten during contraction and elongate during muscle stretch!!!

Summary: During contraction, the I and H bands shorten and the Z-lines move together thus shortening the entire sarcomere. The opposite occurs with stretch or lengthening. The lenght of the A-band never changes. 

 

15

Identify the bands and zones of a sarcomere in this electron micrograph.

16

Identify the stuctures in this electron micrograph of muscle.

17

What are transverse tubules (T-tubules)? Where on the myofibrils are they aligned? What organelle are they closely associated with and why? What is a triad?

T-tubules are tubular invaginations of the sarcolemma and are normally aligned with myofibrils at the A-I band junction. they are closely associated wit the terminal cisternae of the sarcoplasmic reticulum, where Ca2+ ions essential for muscular contraction are stored. The close association of T-tubules and the sarcoplasmic reticulum allow for quick contraction of myofibrils in the muscle fiber despite the fibers' large diameter i.e. allows for changes in the membrane electrical potential to extned into the center of the cell and by virtue of the close linkage with T-tubules, with terminal cisternae of sarcoplasmic reticulum which then release Ca2+

triad: triple structure seen in cross section consisting of 2 terminal cisternae flanking a T-tubule at the 
A-I band jucntion

18

What are the names for the synaptic connection btwn a motor neuron terminal and a muscle?

neuromuscular junction (NMJ)/motor end plate

19

What are junctional folds? What is their purpose? What receptors are located there and what is their ligand? What effect does this ligand have on the muscle?

 folding of the sarcolemma at the NMJ to increase surface area. contains acetylcholine receptors for release of the neurotransmitter aceytlcholine (Ach) from neurons. Ach causes contraction and is responsible for a trophic effect on the target muscle cell. reason why you get muscle atrophy/weakness in diseases involving motor end plate such as myasthenia gravis (autoimmune disease that attacks Ach receptors-when antibody binds them, are internalized and destroyed).

20

What is a motor unit? Where in the body will you find a small vs a large motor unit?

motor unit: a motor neuron (from spinal cord) and all of the muscle fibers it innervates. a motor neuron may branch and innervate a small number of muscle fibers (1 neuron to ~10 fibers) for fine motor control as in the digits of the hand or may have large motor units (1 neuron to ~2000 muscle fibers). large motor units are associated with gross postural control as in erector spinae muscles, abdominal muscles

21

Loss of stimulation to the muscle cell at the NMJ leads to muscle atrophy called _____ atrophy.

dennervation

22

What are muscle spindles? Are they afferent or efferent receptors? What is their structure? What reflex are they the basis for?

muscle spindles are a type of sensory receptor in skeletal muscle that detect the amount of stretch in a muscle (length and rate of length changes). muscle spindles have an outer connective tissue capsule, within which an inner capusle containing 2 types of modified muscle cells (intrafusal fibers) lies. Afferent sensory fibers (Ia afferent) wrapped around intrafusal fibers are stimulated by change in the length of intrafusal fibers in the spindle. efferent fibers modulate the sensitivity of the spindle by adjusting the tension in the intrafusal fibers, thus mantaining sensitivity to stretch during contraction. spindle apparatus is the basis for the afferent limb of the stretch reflex (deep tendon reflex/DTR)

23

Muscle mass is a balance btwn myofibril protein ______ and ______. 

synthesis and degradation

24

During development, myoblasts differentiate from what type of cell?

myotomal cells of somites of the paraxial mesoderm

25

Early myoblasts fuse to form ____ that then mature into skeletal muscle fibers. The _____ (same as previous blank) orginally have centrally located nuclei which then become displaced to the periphery by newly synthesized ____. Differentiation of myoblasts is associated with transcription factors that upregulate ____ (same as previous) gene transcription

myotubes

myofilaments

26

True or false: One can increase the number of muscle fibers (muscle cells) they have with exercise.

False. the number of muscle fibers is determined prenatally

27

What is hypertrophy as it relates to muscle growth and what is the mechanism? How does longitudinal growth of muscles occur with skeletal growth?

1. hypertrophy, or circumfirential muscle growth during development occurs by increasing the size of myofibrils and myofibrils per muscle fiber

2. longitudinal growth occurs via adding new sarcomeres at the ends of myofibrils so that muscle cells increase in length with skeletal growth

28

True or false: Skeletal muscle is adaptive to use throughout life and occurs by changes in fiber size as well as fiber type. 

True. 

29

What are factors affecting hypertrophy?

  • Age: reduced ability to hypertrophy with advancing age

• Nutrition: adequate supply of amino acids necessary
• Hormones: testosterone (and to a lesser extent 
estrogen), growth hormone, FGF and IGF promote 
hypertrophy; cortisol causes muscle breakdown and 
atrophy
• Taking anabolic steroids (including testosterone) as 
performance-enhancing drugs increases hypertrophy (but is illegal without prescription)

30

How do muscle cells regenerate? How well are they able to generate?

satellite cells are responsible for the limited ability of muscle cells to generate and are inside the external lamina of muscle cells. after injury, some become activated, reenter the cell cycle, express myogenic regulator factors, and proliferate. as long as the external lamina remains intact, myoblasts fuse to form myotubes that then mature into a new muscle fiber. if external lamina is disrupted, fibroblasts repair the injured site with resultant scar tissue formation