STUDY MATERIAL AFTER MIDTERM Flashcards
(144 cards)
What range in Nanometers is visible to the human eye?
400nm to 700nm is visible light to the human eye
Fluorescence Definition
Fluorescence
* Fluorescence is the emission of light by a
substance which has absorbed light or other
electromagnetic radiation.
* In most cases, the emitted light has a longer
wavelength, and therefore lower energy, than
the absorbed light.
Why Fluorescence?
Fluorescence allows us to:
- enhance friction ridge details
- eliminate backgrounds
- render latent impressions visible
- increase contrast between impression and background
We achieve fluorescence through the application of chemicals or powders and the use of filters.
Two kinds of filters:
Exiter filter
Barrier filter
Common light sources we use for excitation radiation:
- Ultraviolet (UV) light
- Forensic Light Source (FLS) – filtered lamps
AKA - Alternative Light Source (ALS) - LASER
What PPE is required for Chemicals and Alternative Light sources/
Gloves, long sleeves and Eye Protection
Why do we use Ultraviolet (UV) Light
- Search for hair, fibers, bodily fluids
- Use with fluorescent powders
- Use with dye stain – Ardrox
- Reveal bruises
- Enhance details of bite marks
- Reveal untreated fingerprints on
textured surfaces
Other applications:
* Analysis of inks on questioned
documents
* Counterfeiting detection of bank notes
Goggles MUST have an Optical Density factor of OD___ at 532 nm
or better.
OD4
The most useful light to trigger fluorescence at
crime scenes is blue at approx. _____nm
450
What do we search for with Blue Light?
- Hair, fibres, bodily fluids
- Bones, Teeth
- Bloodstains
- Untreated fingerprints on textured surfaces
- Footwear impressions
- Drugs, Paint, Grease
Some examples Powders and Chemicals we use for Non porous and porous surfaces?
- Non-porous surfaces
- Fluorescent powders
- Cyanoacrylate + dye stain
o Ardrox
o Brilliant Yellow
o Rhodamine 6G - Porous surfaces
- DFO (1,8 Diazafluoren-9-one)
- IND-Zn (1,2-indanedione zinc chloride)
Why use chemicals?
- Eliminate background for better contrast
- Improve chances of finding impressions
- Quality of impressions developed with
chemicals is often better than when
developed with powders
Process we do for non-porous
- Cyanoacrylate fuming
- Fluorescent dyes or fingerprint
powders - Vacuum Metal Deposition (VMD)
CA stands for _____________________________
Cyanoacrylate
Porous Chemical Sequence
1A In/Zn
or
1B DFO
2 Ninhydrin
3 VMD
4 Oil Red O
5 Physical Developer
Wet Porous Chemical Sequence
1 VMD
2 Oil Red O
3 Physical Developer
Non-Porous Chemical Sequence
1 CA
2 CA + Dye
3 VMD
Wet Non-Porous Chemical Sequence
Molybdenum Disulphide/ Small particle Reagent
or
VMD
Semi- Porous Chemical Sequence
1 CA
2 Florescent dyes or fingerprint powders
3 VMD
Why do we use filters?
- to apply light of a specific wave length range for excitation radiation
- to see fluorescence
- to photograph fluorescence
What does LASER stand for?
Light
Amplification by
Stimulated
Emission of
Radiation
What are common problems when using chemicals that fluoresce?
- incorrect match of excitation light and chemical
- no barrier filter on camera
- incorrect barrier filter
- insufficient time for eyes to adapt
What part of the matrix reacts to chemicals?
- eccrine sweat
- sebaceous oils
- lipids
- other contaminants
When using chemicals at a scene what are factors to consider?
- history of the substrate: dry, wet, previously wet
- seriousness of the offense
- availability of chemicals/ powders
- value of item
- potential for damage
- substrate
- matrix
- time elapsed between deposition and examination