Synovial Fluid

Question 1

viscous fluid that lubricates joints

Answer 1

Synovial fluid

Question 1

1. Lubricates joints
2. Reduce friction
3. provides nutrients to articular cartilage
4. Lesson shock of joint compression

Answer 1

Synovial fluid

Question 2

Specialized cells that secretes MUCOPOLYSACCHARIDE that consists of HYALURONIC ACID

Answer 2

Synoviocytes

Question 3

Method of Collection of Synovial Fluid

Answer 3

Arthrocentesis

Question 4

Normal Adult Synovial Fluid

Answer 4

< 3.5 mL

Question 5

Inflammation in joints Synovial fluid

Answer 5

> 25 mL

Question 6

Clotted Synovial Fluid is seen in patient with

Answer 6

Joint Disease

Question 7

Fluid is often collected with ____ that has been moistened with ___

Answer 7

Syringe ; Liquid heparin

Question 8

_____ SHOULD NOT BE USED MAY PRODUCE ARTIFACTS

Answer 8

Powdered anticoagulants

Question 9

Test tubes for Arthrocentesis:

Gram stain and Culture

Answer 9

Sterile heparinized
Sodium Polyanethol sulfonate

Question 10

Test tubes for Arthrocentesis:

Cell count

Answer 10

Liquid EDTA
Heparin

Question 11

Test tubes for Arthrocentesis:

Glucose analysis

Answer 11

Sodium Fluoride

Question 12

Test tubes for Arthrocentesis:

Other test

Answer 12

non-anticoagulated

Question 13

Appearance:

Clear and Pale Yellow

Answer 13

Normal

Question 14

Appearance:

Deeper Yellow

Answer 14

Inflammation

Question 15

Appearance:

Greenish tinge

Answer 15

Infection

Question 16

Appearance:

Red

Answer 16

Trauma/Disorder

Question 17

Appearance:

Milky

Answer 17

Presence crystals

Question 18

Increased Turbidity of Synovial fluid

Answer 18

Rice Bodies
Metal and Plastic (Joint prostheses)
Cartilage Fragment (Orthoarthritis)

Question 19

Presence of Ground Pepper

Answer 19

Ochronosis (Chronotic chards ; Accumulation of homogentisic acid)

Question 20

Polymerization of hyaluronic acid

Answer 20

Viscosity

Question 21

2 test for Synovial Fluid Viscosity

Answer 21

1. String Test
2. Ropes Test / Mucin Clot Test

Question 22

ability to form a string from tip of syringe

Answer 22

String Test

Question 23

Normal String Test

Answer 23

4-6 cm

Question 24

Added in Ropes / Mucin Clot Test

Answer 24

2-5% Acetic Acid

Question 25

In Ropes / Mucin Clot Test Normal Synovial Fluid produces

Answer 25

Solid Clot surrounded by Clear Fluid

Question 26

As the ability of Hyaluronate to polymerize decreases; clots _____ and fluid ____ turbidity

Answer 26

Clot becomes less firm ; Increased Turbidity

Question 27

ROPES / MUCIN CLOT TEST: Solid Clot

Answer 27

Good

Question 28

ROPES / MUCIN CLOT TEST: Soft Clot

Answer 28

Fair

Question 29

ROPES / MUCIN CLOT TEST: Friable Clot

Answer 29

Low

Question 30

ROPES / MUCIN CLOT TEST: No clot

Answer 30

Poor

Question 31

Cell Count for Synovial: Added in very viscous fluid

Answer 31

0.05% Hyaluronidase in phosphate buffer

Question 32

Cell Count for Synovial: Pretreated very viscous synovial fluid is incubated at

Answer 32

37 deg cel. for 5 minutes

Question 33

Cell Count for Synovial: Manual count is done by using

Answer 33

Newbauer Counting chamber

Question 34

Cell Count for Synovial: Clear fluids is usually counted ____ ; while turbid and bloody ____

Answer 34

CLEAR FLUID - UNDILUTED TURBID / BLOODY - DILUTED

Question 35

Cell Count for Synovial: Traditional WBC diluent (Acetic acid) IS NOT USED DUE TO

Answer 35

May form mucin clot

Question 36

Diluents for Synovial Fluid: Total Cell Count, RBC Count

Answer 36

Normal Saline (0.85% NaCl)

Question 37

Diluents for Synovial Fluid: WBC Count

Answer 37

Hypotonic Saline (0.3% NaCl) Saline with saponin

Question 38

Normal Cell Count in Synovial Cells: RBC

Answer 38

< 2000 cells/uL

Question 39

Normal Cell Count in Synovial Cells: WBC

Answer 39

< 200 cells /uL

Question 40

Normal Cell Count in Synovial Cells: Monocytes and Macrophages

Answer 40

65 %

Question 41

Normal Cell Count in Synovial Cells: Lymphocytes

Answer 41

< 25%

Question 42

Normal Cell Count in Synovial Cells: Neutrophils

Answer 42

< 15%