SYNOVIAL FLUID Flashcards

(51 cards)

1
Q

Synovial fluid is also known as?

A

Joint fluid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

It is a viscous liquid found in the cavities of the movable joints (diarthroses) or synovial joints

A

Synovial fluid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Functions of synovial fluid:

A
  1. Lubricate joints
  2. Reduce friction between bones
  3. Provides nutrients to the articular cartilage
  4. Lessen shock of joint compression occurring during activities such as walking and jogging
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Masakit sa arthritis =

A

Watery synovial fluid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

It is the polymerization of hyaluronic acid

A

viscosity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Decrease of synovial fluid viscosity =

A

arthritis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Method of collection for synovial fluid

A

arthrocentesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Normal synovial fluid does not ________

A

Clot (diseased joint may clot)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Synovial fluid

Normal volume in adult knee cavity =
Inflammation =

A

<3.5 mL
>25 mL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

SF:

Colorless - pale yellow (clear) =

A

normal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

SF:

Deeper yellow =

A

inflammation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

SF:

Greenish tinge

A

Bacterial infection

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

SF:

Red =

A

Traumatic tap, hemorrhagic arthritis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

SF:

Turbid =

A

WBCs, synovial cell debris, fibrin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

SF:

Milky =

A

Presence of crystals

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

SF:

Plain red top tube (no anticoagulant) =

A

Chemical and immunologic evaluation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

SF microscopic examination:

Sodium Heparin/ liquid EDTA =

A

Hematology or cell count

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

SF microscopic examination:

Do not use ________ and _________
Do not _______ samples

A

Powdered anticoagulants
Lithium heparin
refrigerate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

SF:

Sterile anticoagulant tube ( heparin or SPS) =

A

Microbiological studies (GS and culture)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Synovial fluid viscosity normal value =

A

Forms a string that is 4-6 cm long

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Test for SF viscosity

A

Ropes or Mucin Clot test (Hyaluronate Polymerization test)

22
Q

Ropes or Mucin clot test:

Reagent =
Positive reaction (hyaluronic acid + acetic acid) =

A

2-5% acetic acid
Mucin clot

23
Q

Grading for SF viscosity:

Good =
Fair =
Low =
Poor =

A

Solid or compact clot, clear solution
Soft clot
Priable clot, cloudy solution
No clot

24
Q

Most frequently performed count in synovial fluid

25
SF WBC count diluting fluids:
NSS with methylene blue Hypotonic saline (0.3%) Saline with saponin
26
Differential count of cells in SF: Rbcs = WBCs = WBC differential =
<2,000/uL < Or = 200/uL 65% = monocytes and macrophages 25% = neutrophils 15% = lymphocytes
27
For every viscous liquid in SF: - add a pinch of hyaluronidase to ____________ or add 1 drop of _______________ in phosphate buffer per mL of fluid - incubate at ________________ for ___________
0.5 mL 0.05% hyaluronidase 37° C 5 min.
28
Cells and inclusions seen in SF: Polymorphonuclear leukocytes = bacterial sepsis, crystal- induced inflammation
Neutrophils (3 lobes)
29
Mononuclear leukocyte = nonseptic inflammation
lymphocyte
30
Large mononuclear monocyte, may be vacuolated = normal, viral infections
macrophages
31
Neutrophils ingested "round body" = lupus erythematosus
LE cell
32
Vacuolated macrophage with ingested neutrophils = reactive arthritis
Reiter cells
33
Neutrophils with dark cytoplasmic granules containing precipitated rheumatoid factor = rheumatoid arthritis, immunologic inflammation
RA cell
34
Morphologically resembles polished rice = tuberculosis, septic, and rheumatoid arthritis
Rice bodies
35
Debris from metal and plastic joint prosthesis "ground pepper appearance" = ochronotic arthropathy, alkaptonuria, ochronosis
Ochronotic shards
36
Large, multinucleated cells = osteoarthritis
Cartilage cells
37
Similar w/ macrophage, but may be multinucleated, resembling a mesothelial cell = normal, disruption from arthrocentesis
Synovial lining cell
38
Refractive intracellular and extracellular globules = traumatic injury, chronic inflammation
Fat droplets
39
Inclusions w/in clusters of synovial cells = pigmented villonodular synovitis
hemosiderin
40
Normal synovial fluid should _________ contain any crystal
not
41
Cause of crystal formation in SF:
1. Metabolic disorders 2. Decreased renal excretion that produces increased blood levels of crystallizing chemicals 3. Degeneration of cartilage and bones 4. Injection of medications (corticosteroid)
42
Most common Needles - birefringe gout
Monosodium urate
43
Rhombic square, rods Most common + Birefringe Pseudogout
Calcium pyrophosphate
44
Notched, rhombic plates - birefringe extracellular
Cholesterol
45
Flat, variable-shaped plates + And - birefringes Injections
Corticosteroid
46
Envelopes - birefringe Renal dialysis
Calcium oxalate
47
Small particles, requires electron microscopy - birefringe Osteoarthritis
apatite
48
Detects the presence or absence of birefringes
Polarizing microscope
49
Confirm the type of birefringence (+ or -) Red compensator is placed between crystal and analyzer
Compensated polarizing microscope
50
- birefringes Yellow color Parallel to long axis
Monosodium urate
51
+ birefringes Blue color Perpendicular to long axis
Calcium pyrophosphate dihydrate (CPPD)