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Flashcards in T lymphocytes Deck (65):
1

What are the three family members of the lymphocytes?

T
B
Natural killer

2

What is meat by the term MHC restriction, in terms of T cells?

T cells are restricted in what they can respond to, since they require an MHC cell to activate them

3

What are the precursors to T cells called? Where are these produced, and where do they go to mature?

Prothymocytes, made in bone marrow, go to Thymus to mature

4

What are the two factors that differentiate T lymphocytes?

Functions and surface proteins

5

Where do T lymphocytes go once they mature in the Thymus/?

To lymphoid organs to await activation by antigen

6

Where are memory T cells found? What do they wait for?

Memory T cells tend to reside in the bloodstream until directed to enter non-lymphoid tissues to elicit cell-mediated immune responses.

7

What are the two subsets of T helper cells?

Th1 and Th2

8

What is the response generated by Th1? Th2?

Th1 = cell-mediated
Th2 = humoral immunity

9

True or false: Th1 cells are exclusive in the fact that they only direct cell mediated immunity

False--there are actually cytokines that Th1 cells produce that stimulate B cells to produce IgG

10

What is the role Th17 plays?

Inflammatory response

11

What is the function of T-follicular helper cells?

promote germinal center formation in lymphoid organs

12

What is the function of cytotoxic T cells?

Lyse virus infected cells

13

Which lymphocytes are responsible for the form of DTH by reacting to noninfectious foreign antigens in the skin and causing a contact dermatitis (like poison ivy)

Cytotoxic T cells

14

What are T regulatory cells?

Cells that secrete cytokines to inhibit immune responses, thus acting to downregulate immunity once a pathogen has been eliminated

15

What are alpha-beta T Cell Antigen Receptor?

the proteins on the surface of T cells that allow them to recognize and respond to antigen

16

How many specific antigens can individual T cells recognize?

One

17

The TCR for most T lymphocytes is comprised of what parts? What binds these parts together?

an alpha and beta heterodimer, bound together by disulfide bridges

18

How are different specific TCRs generated?

Each chain on TCRs are composed of constant and variable regions similar to immunoglobulins

19

There are two chains (alpha and beta) on each TCR. Are both involved in binding to an antigen+MHC protein, or just one?

Both

20

True or false: The TCR is partly responsible for MHC restriction

False--not at all responsible. CD4 and CD3 are.

21

What produces MHC restriction?

CD4 and CD8 proteins

22

The cytoplasmic tails of the TCR chains are not long enough to act as signal transducers (t-rex!!). What transduces the signal from the TCR chains then?

CD3 proteins

23

What are the antigen receptor for B cells?

Antibodies

24

How many antigen bonding sites are on TCRs? B cell surface antibodies?

The TCR has a single antigen binding site, whereas the B cell antigen receptor has two antigen binding sites.

25

Are B cell antibodies secreted? How about the TCR protein on T cells?

B cell antibodies, yes

TCR, no

26

What are CD3 proteins? Are they variable between individuals? What subtypes exits? What sites exist in the tail that allows these proteins to act as signal transducers?

1. Proteins that mediate the response of TCR proteins

2. Invariable

3. 1 gamma, 2 epsilon, and 2 zeta

4. Phosphorylation sites in the tails

27

Besides CD3 proteins, what other protein are needed to transduce a signal from TCR proteins?

Co-stimulatory molecules that stimulate their own pathway.

28

What is the function of CD28? What does it bind to?

co-stimulator of T cells that binds to B7 on macrophages/dendritics cells/others

29

What is CD40 ligand? What does it bind to?

Co-stimulator of T cells tha tbinds to CD40 protein on macrophages/dendritic cells

30

What is the CD4 transmembrane protein? What does it do (3)?

Single polypeptide that is expressed on 65% of T-cells

acts in:
1. cell adhesion
2. Signal transduction in T-cell activation
3. Restricts T-cell response to recognizing only class II MHC

31

Besides T-cells, what other cell types have CD4 proteins? What is the consequence of this?

Macrophages, meaning that AIDS can target marcophages

32

CD4 acts to restrict T cell responses to recognizing only class II MHC proteins. CD4 binds to non-polymorphic (i.e. invariant) regions of class II MHC proteins. Why is this important?

Because CD4 is invariant and wouldn't be able to change to accommodate the many different class II MHC proteins that humans express

33

Most CD8-expressing cells are what cells?

Cytotoxic T cells

34

Structurally, CD8 is composed of what?

A homodimer or heterodimer

35

What are the three functions of CD8 proteins?

1. Cell adhesion to increase the strength of binding between CD8+ T cells and antigen-presenting cells or CTL target cells.

2. signal transducer to aid in T cell activation.

3. Restricts T cell regocnition to only class I MHC proteins

36

When does T cell activation begin?

When TCR binds to an MHC-bound antigen

37

True or false: Clustering of the TCR's with multiple MHC-peptide complexes on an antigen presenting cell has been shown to be important in activating signal transduction

True

38

Through which pathway do TCR proteins activate transcription?

Phospholipase C and MAP kinase cascade

39

What induces transcription of IL-2 cytokine?

Transcription factor AP-1

40

What is IL-2 cytokine?

provides autocrine growth of T cells by transcribing receptor and gene

41

What is the result of IL-2 production in T-cells?

Autcrine stimulation, resulting in mitosis a crap-ton of times

42

What are the two subsets of T helper cells?

Th1 and Th2

43

What directs the production of Th1 vs Th2?

the presence of either interleukin-12 (IL-12) or interleukin-4 (IL-4).

44

What does IL-12 do? What produces it?

Directs T-cells to become Th1 cells.

Macrophages/dendritic cells produce it when they see antigen

45

What does IL-4 do? What produces it?

Directs T-cells to become Th2 cells

Not known what produces it

46

T helper cells are derived from what?

antigen naïve CD4+ T cell precursors (called a Th0 cell),

47

What is the default pathway of helper T-cell maturation?

Th2

48

What is needed for precursors of helper T cells to be directed toward Th1 production?

Inflammation/macrophages etc to produce IL-12

49

Peptides present in large quantities on antigen presenting cells tend to induce (???) responses, whereas those in small amounts on antigen presenting cells elicit (???) responses. (fill in the blanks)

Peptides present in large quantities on antigen presenting cells tend to induce Th1 responses, whereas those in small amounts on antigen presenting cells elicit Th2 responses.

50

Peptides that bind strongly to the T cell antigen receptor cause (???) responses and those that bind weakly produce (???) responses.

Peptides that bind strongly to the T cell antigen receptor cause Th1 responses and those that bind weakly produce Th2 responses.

51

What cytokine do Th1 cells produce? Th2?

Th1- interferon-gamma

Th2- IL-4, IL-5 and IL-13.

52

What does the interferon-gamma that is produced by Th1 cells do? (2)

1. activates the microbicidal activities of macrophages

2. stimulates B cells to produce IgG antibody which can opsonize microbes, thereby enhancing phagocytosis

53

IL-2, together with interferon-gamma, generates what?

cytotoxic T lymphocytes

54

Th2 cytokines promote the production of what?

IgE antibody, and mast cell-mediated immune reactions

55

Why are immune responses generally dominated by either a Th1 or Th2 response?

Th1 will inhibit Th2, and vice versa

56

TH17 cells develop from uncommitted naive T cells when exposed to which cytokines?

IL-1, IL-6 and transforming growth factor-beta (TGF-β)

57

When are IL-1 and IL-6 cytokines produced? What produces them?

produced by dendritic cells during anti-microbial defense and are considered inflammatory cytokines

58

What is the function of IL-23? What cells produce it

Stabilizes TH17 phenotype, produced by dendritic cells

59

CTL typically have what CD protein expressed on the surface? What MHC protein does this recognize?

CD8+, which recognizes class I MHC proteins

60

How are CTLs activated?

The TCR of a pre-CTL binds the peptide/class I MHC complex of a target cell, as well as CD28 (on the CTL) binding the target cell B7 costimulatory proteins

61

What promotes CTLs maturation?

CD4+ Th1-derived interferon-gamma and IL-2

62

There is evidence that CTL subsets exist that can trigger Th1 or Th2 responses. Which response is generated in the less severe form of leprosy? In the more severe form?

Less severe = Th1

More severe = Th2

("They did not choose wisely")

63

What are the five steps involved in CTL killing?

1. TCR- MHC class I binding
2. CTL activation
3. Lethal hit delivered
4. CTL disengage
5. Death of cell

64

What are the two proteins involved in CTL killing, and what is the function of each?

Perforin perforates target cell, allowing for:

Granzyme B entry causes activation of Caspase pathway --->mitochondria release apoptosome

65

What is the Fas ligand?

CTL-expressed receptor that binds to Fas protein, and activates killing